Electron cryo-tomography captures macromolecular complexes in native environments

被引：21
作者：
Baker, Lindsay A. [1 ]
Grange, Michael [1 ]
Grunewald, Kay [1 ]
机构：
[1] Univ Oxford, Wellcome Trust Ctr Human Genet, Oxford Particle Imaging Ctr, Div Struct Biol, Roosevelt Dr, Oxford OX3 7BN, England
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 2017年 / 46卷
基金：
英国惠康基金; 加拿大健康研究院;
关键词：
SITU STRUCTURAL-ANALYSIS; MEMBRANE; ARCHITECTURE; PROTEIN; CRYOTOMOGRAPHY; INFECTION; CELLS; MODEL;
D O I：
10.1016/j.sbi.2017.08.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transmission electron microscopy has a long history in cellular biology. Fixed and stained samples have been used for cellular imaging for over 50 years, but suffer from sample preparation induced artifacts. Electron cryo-tomography (cryoET) instead uses frozen-hydrated samples, without chemical modification, to determine the structure of macromolecular complexes in their native environment. Recent developments in electron microscopes and associated technologies have greatly expanded our ability to visualize cellular features and determine the structures of macromolecular complexes in situ. This review highlights the technological improvements and the new areas of biology these advances have made accessible. We discuss the potential of cryoET to reveal novel and significant biological information on the nanometer or subnanometer scale, and directions for further work.
引用
收藏
页码：149 / 156
页数：8
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