Genetically engineered probiotics

被引:84
作者
Steidler, L
机构
[1] Natl Univ Ireland Univ Coll Cork, Dept Med, Alimentary Pharmabiot Ctr, Cork, Ireland
[2] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Alimentary Pharmabiot Ctr, Cork, Ireland
关键词
genetic engineering; mechanisms of probiotics; Lactobacillus; Lactococcus lactis; in situ delivery; heterologous gene expression; biological containment;
D O I
10.1016/S1521-6918(03)00072-6
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Probiotic micro-organisms have been used for many years. Originating as food supplements, they are now most often administered orally and offer an attractive alternative for treating of intestinal disorders. A better understanding of the mechanisms by which these micro-organisms act has now opened up possibilities for designing new probiotic strains. Through genetic engineering, it is possible not only to strengthen the effects of existing strains, but also to create completely new probiotics. These need not necessarily be composed only of bacterial products but can also include elements of regulatory systems or enzymes derived from a foreign-human-source. If designed carefully and with absolute attention to biological safety in its broadest sense, the development of genetically modified probiotics has the potential to revolutionize alimentary health.
引用
收藏
页码:861 / 876
页数:16
相关论文
共 67 条
[1]  
Anderson M H, 1992, Curr Opin Dent, V2, P157
[2]   Therapy of mucosal candidiasis by expression of an anti-idiotype in human commensal bacteria [J].
Beninati, C ;
Oggioni, MR ;
Boccanera, M ;
Spinosa, MR ;
Maggi, T ;
Conti, S ;
Magliani, W ;
De Bernardis, F ;
Teti, G ;
Cassone, A ;
Pozzi, G ;
Polonelli, L .
NATURE BIOTECHNOLOGY, 2000, 18 (10) :1060-1064
[3]   The 'biodrug' concept: an innovative approach to therapy [J].
Blanquet, S ;
Marol-Bonnin, S ;
Beyssac, E ;
Pompon, D ;
Renaud, M ;
Alric, M .
TRENDS IN BIOTECHNOLOGY, 2001, 19 (10) :393-400
[4]   Use of the alr gene as a food-grade selection marker in lactic acid bacteria [J].
Bron, PA ;
Benchimol, MG ;
Lambert, J ;
Palumbo, E ;
Deghorain, M ;
Delcour, J ;
de Vos, WM ;
Kleerebezem, M ;
Hols, P .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (11) :5663-5670
[5]   L-(+)-LACTATE DEHYDROGENASE-DEFICIENCY IS LETHAL IN STREPTOCOCCUS-MUTANS [J].
CHEN, AP ;
HILLMAN, JD ;
DUNCAN, M .
JOURNAL OF BACTERIOLOGY, 1994, 176 (05) :1542-1545
[6]   Vaginal immunization of Cynomolgus monkeys with Streptococcus gordonii expressing HIV-1 and HPV 16 antigens [J].
Di Fabio, S ;
Medaglini, D ;
Rush, CM ;
Corrias, F ;
Panzini, GL ;
Pace, M ;
Verani, P ;
Pozzi, G ;
Titti, F .
VACCINE, 1998, 16 (05) :485-492
[7]   UNIVERSAL BARRIER TO LATERAL SPREAD OF SPECIFIC GENES AMONG MICROORGANISMS [J].
DIAZ, E ;
MUNTHALI, M ;
DELORENZO, V ;
TIMMIS, KN .
MOLECULAR MICROBIOLOGY, 1994, 13 (05) :855-861
[8]   Oral treatment with Lactococcus lactis expressing Staphylococcus hyicus lipase enhances lipid digestion in pigs with induced pancreatic insufficiency [J].
Drouault, S ;
Juste, C ;
Marteau, P ;
Renault, P ;
Corthier, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (06) :3166-3168
[9]  
Drouault S, 1999, APPL ENVIRON MICROB, V65, P4881
[10]   Recombinant human interleukin 10 in the treatment of patients with mild to moderately active Crohn's disease [J].
Fedorak, RN ;
Gangl, A ;
Elson, CO ;
Rutgeerts, P ;
Schreiber, S ;
Wild, G ;
Hanauer, SB ;
Kilian, A ;
Cohard, M ;
LeBeaut, A ;
Feagan, B .
GASTROENTEROLOGY, 2000, 119 (06) :1473-1482