Dithiobissuccinimidyl propionate self assembled monolayer based cholesterol biosensor

被引：27
作者：
Arya, Sunil K.
Pandey, Pratibha
Singh, Surinder P.
Datta, Monika
Malhotra, Bansi D.
机构：
[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India
[2] Univ Delhi, Dept Chem, Delhi 110007, India
来源：
ANALYST | 2007年 / 132卷 / 10期
关键词：
D O I：
10.1039/b707000d
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A dithiobissuccinimidyl propionate (DTSP) self-assembled monolayer (SAM) prepared onto a gold (Au) surface has been utilized for covalent immobilization of cholesterol oxidase (ChOx) and cholesterol esterase (ChEt). These ChOx-ChEt/DTSP/Au bio-electrodes have been characterized using electrochemical impedance and cyclic voltammetric (CV) techniques, respectively. Differential pulse voltammetry (DPV) has been used for enzymatic assay of immobilized ChOx and ChEt onto the DTSP modified gold surface as a function of cholesterol oleate concentration. The response measurement conducted on ChOx-ChEt/DTSP/Au bio-electrode reveal the value of Michaelis-Menten constant ( Km) as 0.95 mM suggesting enhanced affinity of enzymes (ChOx and ChEt). The ChOx-ChEt/DTSP/Au bio-electrodes show linearity in range of 50 to 400 mg dl(-1) of cholesterol oleate and the shelf-life of more than 50 days when stored at 4 degrees C. This biosensing electrode shows correlation coefficient of 0.9973 and standard deviation of regression as 0.859 mA.
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页码：1005 / 1009
页数：5
相关论文
共 36 条
[1]   Sensitive estimation of total cholesterol in blood using Au nanowires based micro-fluidic platform [J].
Aravamudhan, Shyam ;
Kumar, Arun ;
Mohapatra, Shyam ;
Bhansali, Shekhar .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (9-10) :2289-2294
[2]   Cholesterol biosensor based on N-(2-aminoethyl)-3-aminopropyl-trimethoxysilane self-assembled monolayer [J].
Arya, Sunil K. ;
Prusty, Arun K. ;
Singh, S. P. ;
Solanki, Pratima R. ;
Pandey, Manoj K. ;
Datta, Monika ;
Malhotra, Bansi D. .
ANALYTICAL BIOCHEMISTRY, 2007, 363 (02) :210-218
[3]   Poly-(3-hexylthiophene) self-assembled monolayer based cholesterol biosensor using surface plasmon resonance technique [J].
Arya, Sunil K. ;
Solanki, Pratima R. ;
Singh, S. P. ;
Kaneto, K. ;
Pandey, Manoj K. ;
Datta, Monika ;
Malhotra, Bansi D. .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (11) :2516-2524
[4]   Application of octadecanethiol self-assembled monolayer to cholesterol biosensor based on surface plasmon resonance technique [J].
Arya, Sunil K. ;
Solanki, Pratima R. ;
Singh, Ravindra P. ;
Pandey, Manoj K. ;
Datta, Monika ;
Malhotra, Bansi D. .
TALANTA, 2006, 69 (04) :918-926
[5]   Steady-state oxidation of cholesterol catalyzed by cholesterol oxidase in lipid bilayer membranes on platinum electrodes [J].
Bokoch, MP ;
Devadoss, A ;
Palencsár, MS ;
Burgess, JD .
ANALYTICA CHIMICA ACTA, 2004, 519 (01) :47-55
[6]   An electrochemical multienzymatic biosensor for determination of cholesterol [J].
Bongiovanni, C ;
Ferri, T ;
Poscia, A ;
Varalli, M ;
Santucci, R ;
Desideri, A .
BIOELECTROCHEMISTRY, 2001, 54 (01) :17-22
[7]   Anti-rabbit immunoglobulin G detection in complex medium by PM-RAIRS and QCM Influence of the antibody immobilisation method [J].
Briand, Elisabeth ;
Salmain, Michele ;
Compere, Chantal ;
Pradier, Claire-Marie .
BIOSENSORS & BIOELECTRONICS, 2007, 22 (12) :2884-2890
[8]   N-hydroxysuccinimide-terminated self-assembled monolayers on gold for biomolecules immobilisation [J].
Cabrita, JF ;
Abrantes, LM ;
Viana, AS .
ELECTROCHIMICA ACTA, 2005, 50 (10) :2117-2124
[9]   An integrated bienzyme glucose oxidase-fructose dehydrogenase-tetrathiafulvalene-3-mercaptopropionic acid-gold electrode for the simultaneous determination of glucose and fructose [J].
Campuzano, S ;
Loaiza, SA ;
Pedrero, M ;
de Villena, FJM ;
Pingarrón, JM .
BIOELECTROCHEMISTRY, 2004, 63 (1-2) :199-206
[10]   Dithiobissuccinimidyl propionate as an anchor for assembling peroxidases at electrodes surfaces and its application in a H2O2 biosensor [J].
Darder, M ;
Takada, K ;
Pariente, F ;
Lorenzo, E ;
Abruña, HD .
ANALYTICAL CHEMISTRY, 1999, 71 (24) :5530-5537
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