Gunn rats with glial activation in the hippocampus show prolonged immobility time in the forced swimming test and tail suspension test

被引：40

作者：

Arauchi, Ryosuke ^{[1
]}

Hashioka, Sadayuki ^{[1
]}

Tsuchie, Keiko ^{[1
]}

Miyaoka, Tsuyoshi ^{[1
]}

Tsumori, Toshiko ^{[2
]}

Limoa, Erlyn ^{[1
,3
]}

Azis, Ilhamuddin A. ^{[1
,3
]}

Oh-Nishi, Arata ^{[1
]}

Miura, Shoko ^{[1
]}

Otsuki, Koji ^{[1
]}

Kanayama, Misako ^{[1
]}

Izuhara, Muneto ^{[1
]}

Nagahama, Michiharu ^{[1
]}

Kawano, Kiminori ^{[1
]}

Araki, Tomoko ^{[1
]}

Liaury, Kristian ^{[3
]}

Abdullah, Rostia A. ^{[1
,3
]}

Wake, Rei ^{[1
]}

Hayashida, Maiko ^{[1
]}

Inoue, Ken ^{[4
]}

Horiguchi, Jun ^{[1
]}

机构：

[1] Shimane Univ, Dept Psychiat, Izumo, Shimane, Japan

[2] Prefectural Univ Hiroshima, Dept Nursing, Mihara, Japan

[3] Hasanuddin Univ, Dept Psychiat, Makassar, South Sulawesi, Indonesia

[4] Kochi Univ, Hlth Serv Ctr, Kochi, Japan

来源：

BRAIN AND BEHAVIOR | 2018年 / 8卷 / 08期

基金：

日本学术振兴会;

关键词：

astrocytes; forced swimming test; Gunn rat; hippocampus; microglia; tail suspension test; MAJOR DEPRESSIVE DISORDER; PREFRONTAL CORTEX; MEMORY IMPAIRMENT; MICROGLIA; SCHIZOPHRENIA; ASTROCYTE; NEUROINFLAMMATION; PATHOLOGY; BEHAVIOR; BRAIN;

D O I：

10.1002/brb3.1028

中图分类号：

B84 [心理学]; C [社会科学总论]; Q98 [人类学];

学科分类号：

03 ; 0303 ; 030303 ; 04 ; 0402 ;

摘要：

Introduction: Recent studies imply that glial activation plays a role in the pathogenesis of psychiatric disorders, such as schizophrenia and major depression. We previously demonstrated that Gunn rats with hyperbilirubinemia show congenital gliosis and schizophrenia-like behavior. Methods: As it has been suggested that major depression involves glial activation associated with neuroinflammation, we examined whether Gunn rats show depression-like behavior using the forced swimming test (FST) and the tail suspension test (TST). In addition, we quantitatively evaluated both microgliosis and astrogliosis in the hippocampus of Gunn rats using immunohistochemistry analysis of the microglial marker ionized calcium-binding adaptor molecule (Iba) 1 and the astrocytic marker S100B. Results: Both the FST and TST showed that immobility time of Gunn rats was significantly longer than that of normal control Wistar rats, indicating that Gunn rats are somewhat helpless, a sign of depression-like behavior. In the quantification of immunohistochemical analysis, Iba1immunoreactivity in the dentate gyrus (DG), cornu ammonis (CA) 1, and CA3 and the number of Iba1-positive cells in the CA1 and CA3 were significantly increased in Gunn rats compared to Wistar rats. S100B immunore-activity in the DG, CA1, and CA3 and the number of S100B-positive cells in the DG and CA3 were significantly increased in Gunn rats compared to Wistar rats. Conclusion: Our findings suggest that both microglia and astrocyte are activated in Gunn rats and their learned helplessness could be related to glial activation.

引用

页数：9

共 46 条

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