Identification, characterization and expression analysis of transient receptor potential channel genes in the oriental fruit fly, Bactrocera dorsalis

被引:20
作者
Su, Hong-ai [1 ]
Bai, Xue [1 ]
Zeng, Tian [1 ]
Lu, Yong-yue [1 ]
Qi, Yi-xiang [1 ]
机构
[1] South China Agr Univ, Coll Agr, Dept Entomol, Wushan Rd 483, Guangzhou 510642, Guangdong, Peoples R China
来源
BMC GENOMICS | 2018年 / 19卷
关键词
Bactrocera dorsalis; Transient receptor potential; Cationic channels; Splice forms; Expression analysis; DROSOPHILA TRP CHANNELS; FUNCTIONAL-CHARACTERIZATION; LARVAL; TEPHRITIDAE; ANTENNAE; NEURONS; DIPTERA; FLIES; GAMMA; NOMPC;
D O I
10.1186/s12864-018-5053-7
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: Members of the transient receptor potential (TRP) superfamily are proteins that are critical for insects to detect changes in environmental stimuli and also play key roles in their sensory physiology. Moreover, this family provides potential targets for the design of insecticides. In contrast to a large number of studies conducted on Drosophila melanogaster, molecular studies to characterize TRP channels in agricultural pests are lacking. Results: In this study, we identified 15 TRP channel genes in the genome of a notorious agricultural pest, the oriental fruit fly (Bactrocera dorsalis). Comparative analysis of the TRP channels (TRPs) in B. dorsalis with those in D. melanogaster, Glossina morsitans, Musca domestica and the closely related Ceratitis capitata, and TRPs from mosquitoes, Hymenoptera, Lepidoptera, Coleoptera and Hemiptera reveals that members of TRPA and TRPP subfamily are most diverse among insects. The results also suggest that Tephritidae family have two TRP-Polycystin 2 members even though most insects either possess just one or none. The highest expression levels of these two genes are in the testes of B. dorsalis, implying a role in regulating sperm function. We analyzed the expression profiles of the TRP channels identified in this study at different life stages using quantitative real time PCR. The results of this study demonstrate that all TRP channels are mainly expressed in adults, especially at mature stages. The one exception to this trend is BdTRPM, which is more highly expressed in the eggs of B. dorsalis, implying an important role in early development We also detected the spatial expression of TRP channels in mature adult fruit flies by investigating expression levels within various tissues including those involved in sensory function, such as antennae, compound eyes, mouthparts, legs, and wings, as well as tissues critical for homeostasis and physiology (i.e., Malpighian tubules, the brain and gut as well as fat bodies, ovaries, and testes). Conclusion: The results of this study establish a solid foundation for future functional characterization of B. dorsalis TRP channels as well as those of other insects and will help future insecticide design targeting these channels.
引用
收藏
页数:13
相关论文
共 50 条
  • [41] Molecular characterizations of natalisin and its roles in modulating mating in the oriental fruit fly, Bactrocera dorsalis (Hendel)
    Gui, S. -H.
    Jiang, H. -B.
    Liu, X. -Q.
    Xu, L.
    Wang, J. -J.
    INSECT MOLECULAR BIOLOGY, 2017, 26 (01) : 103 - 112
  • [42] Larval competition within and between insecticide resistant and susceptible individuals in the oriental fruit fly, Bactrocera dorsalis
    Okuyama, T.
    Hsu, J. -C.
    JOURNAL OF APPLIED ENTOMOLOGY, 2013, 137 (04) : 289 - 295
  • [43] Influence of Host Shift on Genetic Differentiation of the Oriental Fruit Fly,Bactrocera dorsalis
    WAN Xuan-wu
    LIU Ying-hong
    LUO Lin-ming
    FENG Chuan-hong
    WANG Sheng
    MA Li
    Journal of Integrative Agriculture, 2014, 13 (12) : 2701 - 2708
  • [44] Adipokinetic hormone receptor gene identification and its role in triacylglycerol mobilization and sexual behavior in the oriental fruit fly (Bactrocera dorsalis)
    Hou, Qiu-Li
    Chen, Er-Hu
    Jiang, Hong-Bo
    Wei, Dan-Dan
    Gui, Shun-Hua
    Wang, Jin-Jun
    Smagghe, Guy
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2017, 90 : 1 - 13
  • [45] cDNA isolation, expression, and hormonal regulation of yolk protein genes in the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)
    Chen, Shiu-Ling
    Lin, Chung-Pin
    Lu, Kuang-Hui
    JOURNAL OF INSECT PHYSIOLOGY, 2012, 58 (06) : 763 - 770
  • [46] Genome-wide identification of chitinase and chitin deacetylase gene families in the oriental fruit fly, Bactrocera dorsalis (Hendel)
    Liu, Shi-Huo
    Li, Hong-Fei
    Yang, Yang
    Yang, Rui-Lin
    Yang, Wen-Jia
    Jiang, Hong-Bo
    Dou, Wei
    Smagghe, Guy
    Wang, Jin-Jun
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2018, 27 : 13 - 22
  • [47] Endosymbiotic Bacteria Aid to Overcome Temperature Induced Stress in the Oriental Fruit Fly, Bactrocera dorsalis
    Ayyasamy, Arthikirubha
    Kempraj, Vivek
    Pagadala Damodaram, Kamala Jayanthi
    MICROBIAL ECOLOGY, 2021, 82 (03) : 783 - 792
  • [48] MOLECULAR CHARACTERIZATION OF THE CDNA ENCODING ECDYSONE RECEPTOR ISOFORM B1 AND ITS EXPRESSION IN THE ORIENTAL FRUIT FLY, BACTROCERA DORSALIS (DIPTERA: TEPHRITIDAE)
    Cong, Lin
    Yang, Wen-Jia
    Shen, Guang-Mao
    Dou, Wei
    Wang, Jin-Jun
    FLORIDA ENTOMOLOGIST, 2012, 95 (03) : 650 - 658
  • [49] Inferences on the population structure and colonization process of the invasive oriental fruit fly, Bactrocera dorsalis (Mendel)
    Aketarawong, N.
    Bonizzoni, M.
    Thanaphum, S.
    Gomulski, L. M.
    Gasperi, G.
    Malacrida, A. R.
    Gugliemino, C. R.
    MOLECULAR ECOLOGY, 2007, 16 (17) : 3522 - 3532
  • [50] Genome-wide identification, phylogenetic analysis, and expression profiles of ATP-binding cassette transporter genes in the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)
    Xiao, Lin-Fan
    Zhang, Wei
    Jing, Tian-Xing
    Zhang, Meng-Yi
    Miao, Ze-Qing
    Wei, Dan-Dan
    Yuan, Guo-Rui
    Wang, Jin-Jun
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS, 2018, 25 : 1 - 8