共 31 条
Experimental free energy surface reconstruction from single-molecule force spectroscopy using Jarzynski's equality
被引:161
作者:

Harris, Nolan C.
论文数: 0 引用数: 0
h-index: 0
机构:
Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA

Song, Yang
论文数: 0 引用数: 0
h-index: 0
机构:
Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA

Kiang, Ching-Hwa
论文数: 0 引用数: 0
h-index: 0
机构:
Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA
机构:
[1] Rice Univ, Dept Phys & Astron, Houston, TX 77005 USA
关键词:
D O I:
10.1103/PhysRevLett.99.068101
中图分类号:
O4 [物理学];
学科分类号:
0702 ;
摘要:
We used the atomic force microscope to manipulate and unfold individual molecules of the titin I27 domain and reconstructed its free energy surface using Jarzynski's equality. The free energy surface for both stretching and unfolding was reconstructed using an exact formula that relates the nonequilibrium work fluctuations to the molecular free energy. In addition, the unfolding free energy barrier, i.e., the activation energy, was directly obtained from experimental data for the first time. This Letter demonstrates that Jarzynski's equality can be used to analyze nonequilibrium single-molecule experiments, and to obtain the free energy surfaces for molecular systems, including interactions for which only nonequilibrium work can be measured.
引用
收藏
页数:4
相关论文
共 31 条
[1]
A simple method for probing the mechanical unfolding pathway of proteins in detail
[J].
Best, RB
;
Fowler, SB
;
Toca-Herrera, JL
;
Clarke, J
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2002, 99 (19)
:12143-12148

Best, RB
论文数: 0 引用数: 0
h-index: 0
机构:
Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England

Fowler, SB
论文数: 0 引用数: 0
h-index: 0
机构:
Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England

Toca-Herrera, JL
论文数: 0 引用数: 0
h-index: 0
机构:
Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England

Clarke, J
论文数: 0 引用数: 0
h-index: 0
机构:
Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England Med Res Council Ctr Protein Engn, Dept Chem, Cambridge CB2 1EW, England
[2]
Mechanical processes in biochemistry
[J].
Bustamante, C
;
Chemla, YR
;
Forde, NR
;
Izhaky, D
.
ANNUAL REVIEW OF BIOCHEMISTRY,
2004, 73
:705-748

Bustamante, C
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Chemla, YR
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Forde, NR
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Izhaky, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[3]
Mechanical and chemical unfolding of a single protein: A comparison
[J].
Carrion-Vazquez, M
;
Oberhauser, AF
;
Fowler, SB
;
Marszalek, PE
;
Broedel, SE
;
Clarke, J
;
Fernandez, JM
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1999, 96 (07)
:3694-3699

Carrion-Vazquez, M
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Oberhauser, AF
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Fowler, SB
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Marszalek, PE
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Broedel, SE
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

论文数: 引用数:
h-index:
机构:

Fernandez, JM
论文数: 0 引用数: 0
h-index: 0
机构:
Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
[4]
Verification of the Crooks fluctuation theorem and recovery of RNA folding free energies
[J].
Collin, D
;
Ritort, F
;
Jarzynski, C
;
Smith, SB
;
Tinoco, I
;
Bustamante, C
.
NATURE,
2005, 437 (7056)
:231-234

Collin, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Ritort, F
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Jarzynski, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Smith, SB
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Tinoco, I
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA

Bustamante, C
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[5]
Intrinsic rates and activation free energies from single-molecule pulling experiments
[J].
Dudko, OK
;
Hummer, G
;
Szabo, A
.
PHYSICAL REVIEW LETTERS,
2006, 96 (10)

Dudko, OK
论文数: 0 引用数: 0
h-index: 0
机构:
NIH, Math & Stat Comp Lab, Div Computat Biosci, Ctr Informat Technol, Bethesda, MD 20892 USA NIH, Math & Stat Comp Lab, Div Computat Biosci, Ctr Informat Technol, Bethesda, MD 20892 USA

Hummer, G
论文数: 0 引用数: 0
h-index: 0
机构: NIH, Math & Stat Comp Lab, Div Computat Biosci, Ctr Informat Technol, Bethesda, MD 20892 USA

Szabo, A
论文数: 0 引用数: 0
h-index: 0
机构: NIH, Math & Stat Comp Lab, Div Computat Biosci, Ctr Informat Technol, Bethesda, MD 20892 USA
[6]
RNA structure - Pulling on hair(pins)
[J].
Fernandez, JM
;
Chu, S
;
Oberhauser, AF
.
SCIENCE,
2001, 292 (5517)
:653-654

Fernandez, JM
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Chu, S
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA

Oberhauser, AF
论文数: 0 引用数: 0
h-index: 0
机构: Mayo Clin & Mayo Fdn, Dept Physiol & Biophys, Rochester, MN 55905 USA
[7]
Using nonequilibrium measurements to determine macromolecule free-energy differences
[J].
Fox, RF
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2003, 100 (22)
:12537-12538

Fox, RF
论文数: 0 引用数: 0
h-index: 0
机构:
Georgia Inst Technol, Sch Phys, Atlanta, GA 30332 USA Georgia Inst Technol, Sch Phys, Atlanta, GA 30332 USA
[8]
Biological physics
[J].
Frauenfelder, H
;
Wolynes, PG
;
Austin, RH
.
REVIEWS OF MODERN PHYSICS,
1999, 71 (02)
:S419-S430

Frauenfelder, H
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif Los Alamos Natl Lab, Ctr Nonlinear Studies, Los Alamos, NM 87545 USA Univ Calif Los Alamos Natl Lab, Ctr Nonlinear Studies, Los Alamos, NM 87545 USA

Wolynes, PG
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Los Alamos Natl Lab, Ctr Nonlinear Studies, Los Alamos, NM 87545 USA

Austin, RH
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Los Alamos Natl Lab, Ctr Nonlinear Studies, Los Alamos, NM 87545 USA
[9]
Autoionization in liquid water
[J].
Geissler, PL
;
Dellago, C
;
Chandler, D
;
Hutter, J
;
Parrinello, M
.
SCIENCE,
2001, 291 (5511)
:2121-2124

Geissler, PL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

Dellago, C
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

Chandler, D
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

Hutter, J
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

Parrinello, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA
[10]
Mechanisms of protein folding
[J].
Grantcharova, V
;
Alm, EJ
;
Baker, D
;
Horwich, AL
.
CURRENT OPINION IN STRUCTURAL BIOLOGY,
2001, 11 (01)
:70-82

Grantcharova, V
论文数: 0 引用数: 0
h-index: 0
机构: Univ Washington, Dept Biochem, Seattle, WA 98195 USA

Alm, EJ
论文数: 0 引用数: 0
h-index: 0
机构: Univ Washington, Dept Biochem, Seattle, WA 98195 USA

Baker, D
论文数: 0 引用数: 0
h-index: 0
机构: Univ Washington, Dept Biochem, Seattle, WA 98195 USA

Horwich, AL
论文数: 0 引用数: 0
h-index: 0
机构: Univ Washington, Dept Biochem, Seattle, WA 98195 USA