TLR4-mediated skin carcinogenesis is dependent on immune and radioresistant cells

被引:144
作者
Mittal, Deepak [1 ]
Saccheri, Fabiana [1 ]
Venereau, Emilie [2 ]
Pusterla, Tobias [2 ]
Bianchi, Marco E. [2 ]
Rescigno, Maria [1 ]
机构
[1] European Inst Oncol, Dept Expt Oncol, I-20141 Milan, Italy
[2] San Raffaele Univ & Res Inst, Dept Genet & Cell Biol, Milan, Italy
关键词
HMGB1; inflammation; skin; TLR4; tumourigenesis; TOLL-LIKE RECEPTOR-4; MOBILITY GROUP BOX-1; GLYCATION END-PRODUCTS; CHEMICAL CARCINOGENESIS; TUMOR-DEVELOPMENT; INFLAMMATION; CANCER; HMGB1; PROTEIN; RAGE;
D O I
10.1038/emboj.2010.94
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Skin cancers are the most commonly diagnosed cancers. Understanding what are the factors contributing to skin tumour development can be instrumental to identify preventive therapies. The myeloid differentiation primary response gene (MyD) 88, the downstream adaptor protein of most Toll-like receptors (TLR), has been shown to be involved in seve ral mouse tumourigenesis models. We show here that TLR4, but not TLR2 or TLR9, is upstream of MyD88 in skin tumourigenesis. TLR4 triggering is not dependent on lipopolysaccharide associated to skin-colonizing bacteria, but on the high mobility group box-1 protein (HMGB1), an endogenous ligand of TLR4. HMGB1 is released by necrotic keratinocytes and is required for the recruitment of inflammatory cells and for the initiation of inflammation. The expression of TLR4 on both bone marrow-derived and radioresistant cells is necessary for carcinogenesis. Consistently, a human tissue microarray analysis showed that melanoma and colon cancer display an over-expression of TLR4 and its downstream adaptor protein MyD88 within tumours. Together, our results suggest that the initial release of HMGB1 triggers a TLR4-dependent inflammatory response that leads to tumour development. The EMBO Journal (2010) 29, 2242-2252. doi:10.1038/emboj.2010.94; Published online 4 June 2010
引用
收藏
页码:2242 / 2252
页数:11
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