Azide-alkyne coupling:: A powerful reaction for bioconjugate chemistry

被引：190

作者：

Breinbauer, R ^{[1
]}

Köhn, M

机构：

[1] Univ Dortmund, Fachbereich 3, D-44221 Dortmund, Germany

[2] Max Planck Inst Mol Physiol, Dept Biol Chem, D-44227 Dortmund, Germany

来源：

CHEMBIOCHEM | 2003年 / 4卷 / 11期

关键词：

Alkynes; Azides; Bioconjugates; Bioorganic chemistry; Chemical biology;

D O I：

10.1002/cbic.200300705

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tying together biological molecules is a challenge for molecular biologists and chemists. The azide-alkyne [3+2] cycloaddition has recently been introduced as a highly chemoselective ligation reaction in chemical biology. It has found application in the preparation of carbohydrate arrays, in the modification of viruses such as the capsid of the cowpea mosaic virus (CPMV; see scheme), and in the activity-based protein profiling of whole cells.

引用

页码：1147 / 1149

页数：3

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