Analytical and clinical performance of a Chikungunya qRT-PCR for Central and South America

被引：17

作者：

Edwards, Thomas ^{[1
]}

Castillo Signor, Leticia del Carmen ^{[2
]}

Williams, Christopher ^{[1
]}

Larcher, Clement ^{[3
]}

Espinel, Mauricio ^{[4
]}

Theaker, Jane ^{[3
,5
]}

Donis, Evelin ^{[2
]}

Cuevas, Luis E. ^{[1
]}

Adams, Emily R. ^{[1
]}

机构：

[1] Univ Liverpool Liverpool Sch Trop Med, Res Ctr Drugs & Diagnost, Liverpool, Merseyside, England

[2] Minist Salud Publ & Asistencia Social Guatemala, Lab Nacl Salad Guatemala, Guatemala City, Guatemala

[3] QIAGEN Manchester Ltd, Skelton House,Lloyd St North, Manchester, Lancs, England

[4] Univ San Francisco Quito, Quito, Ecuador

[5] LGC, Darwin House,Faraday St,Birchwood Pk, Risley, Cheshire, England

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2017年 / 89卷 / 01期

关键词：

Diagnostics; Chikungunya; Arboviruses; Molecular diagnostics; TIME RT-PCR; VIRUS; DENGUE; ASSAY;

D O I：

10.1016/j.diagmicrobio.2017.06.001

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Chikungunya was introduced into the Americas in 2015 causing a pandemic across the continent. Testing during the acute phase of infection relies on qRT-PCR, but available assays have a number of limitations. A qRT-PCR assay specific to the chikungunya El gene was designed using sequence data from contemporary strains. A probit analysis established the 95% limit of detection as 19.6 copies per reaction. We compared the assay with a US Centers for Disease Control (CDC) chilcungunya qRT-PCR as the reference standard. The assay had a sensitivity and specificity of 98.4% and 100%in 90 samples retrospectively collected in Guatemala. In a further 74 febrile samples prospectively collected in Ecuador and Guatemala the test had a sensitivity and specificity of 100% and 98.4%, respectively. Sequencing the nsp4 gene of the discordant positive sample indicated the presence of chikungunya RNA, and mismatches to the primer binding sites of the CDC assay. (C) 2017 The Authors. Published by Elsevier Inc.

引用

页码：35 / 39

页数：5

共 22 条

[1] Viremic profiles in asymptomatic and symptomatic chikungunya fever: a blood transfusion threat? [J].

Appassakij, Hatsadee ;

Khuntikij, Paiwon ;

Kemapunmanus, Marisa ;

Wutthanarungsan, Rochana ;

Silpapojakul, Khachornsakdi .

TRANSFUSION, 2013, 53 (10) :2567-2574

[2] Development of a Multi-Target TaqMan Assay to Detect Eastern Equine Encephalitis Virus Variants in Mosquitoes [J].

Armstrong, Philip M. ;

Prince, Nicholanna ;

Andreadis, Theodore G. .

VECTOR-BORNE AND ZOONOTIC DISEASES, 2012, 12 (10) :872-876

[3] Primer Sequence Disclosure: A Clarification of the MIQE Guidelines [J].

Bustin, Stephen A. ;

Benes, Vladimir ;

Garson, Jeremy A. ;

Hellemans, Jan ;

Huggett, Jim ;

Kubista, Mikael ;

Mueller, Reinhold ;

Nolan, Tania ;

Pfaffl, Michael W. ;

Shipley, Gregory L. ;

Vandesompele, Jo ;

Wittwer, Carl T. .

CLINICAL CHEMISTRY, 2011, 57 (06) :919-921

[4] Guillain-Barre Syndrome outbreak associated with Zika virus infection in French Polynesia: a case-control study [J].

Cao-Lormeau, Van-Mai ;

Blake, Alexandre ;

Mons, Sandrine ;

Lastere, Stephane ;

Roche, Claudine ;

Vanhomwegen, Jessica ;

Dub, Timothee ;

Baudouin, Laure ;

Teissier, Anita ;

Larre, Philippe ;

Vial, Anne-Laure ;

Decam, Christophe ;

Choumet, Valerie ;

Halstead, Susan K. ;

Willison, Hugh J. ;

Musset, Lucile ;

Manuguerra, Jean-Claude ;

Despres, Philippe ;

Fournier, Emmanuel ;

Mallet, Henri-Pierre ;

Musso, Didier ;

Fontanet, Arnaud ;

Neil, Jean ;

Ghawche, Frederic .

LANCET, 2016, 387 (10027) :1531-1539

[5] Development of a multiplex real-time RT-PCR assay for simultaneous detection of dengue and chikungunya viruses [J].

Cecilia, D. ;

Kakade, M. ;

Alagarasu, K. ;

Patil, J. ;

Salunke, A. ;

Parashar, D. ;

Shah, P. S. .

ARCHIVES OF VIROLOGY, 2015, 160 (01) :323-327

[6] Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014 [J].

Chiu, Charles Y. ;

Bres, Vanessa ;

Yu, Guixia ;

Krysztof, David ;

Naccache, Sarnia N. ;

Lee, Deanna ;

Pfeil, Jacob ;

Linnen, Jeffrey M. ;

Stramer, Susan L. .

EMERGING INFECTIOUS DISEASES, 2015, 21 (08) :1409-1413

[7] The Brazilian Zika virus strain causes birth defects in experimental models [J].

Cugola, Fernanda R. ;

Fernandes, Isabella R. ;

Russo, Fabiele B. ;

Freitas, Beatriz C. ;

Dias, Joao L. M. ;

Guimaraes, Katia P. ;

Benazzato, Cecilia ;

Almeida, Nathalia ;

Pignatari, Graciela C. ;

Romero, Sarah ;

Polonio, Carolina M. ;

Cunha, Isabela ;

Freitas, Carla L. ;

Brandao, Wesley N. ;

Rossato, Cristiano ;

Andrade, David G. ;

Faria, Daniele de P. ;

Garcez, Alexandre T. ;

Buchpigel, Carlos A. ;

Braconi, Carla T. ;

Mendes, Erica ;

Sall, Amadou A. ;

Zanotto, Paolo M. de A. ;

Peron, Jean Pierre S. ;

Muotri, Alysson R. ;

Beltrao-Braga, Patricia C. B. .

NATURE, 2016, 534 (7606) :267-+

[8] Molecular diagnosis and analysis of Chikungunya virus [J].

Edwards, Carolyn J. ;

Welch, Stephen R. ;

Chamberlain, John ;

Hewson, Roger ;

Tolley, Howard ;

Cane, Patricia A. ;

Lloyd, Graham .

JOURNAL OF CLINICAL VIROLOGY, 2007, 39 (04) :271-275

[9] Prospective detection of chikungunya virus in blood donors, Caribbean 2014 [J].

Gallian, Pierre ;

de Lamballerie, Xavier ;

Salez, Nicolas ;

Piorkowski, Geraldine ;

Richard, Pascale ;

Paturel, Laure ;

Djoudi, Rachid ;

Leparc-Goffart, Isabelle ;

Tiberghien, Pierre ;

Chiaroni, Jaques ;

Charrel, Remi N. .

BLOOD, 2014, 123 (23) :3679-+

[10]

Ganu Mohini A, 2011, J Assoc Physicians India, V59, P83

← 1 2 3 →