A novel cysteine protease inhibitor with lectin activity from the epidermis of the Japanese eel Anguilla japonica

被引：14

作者：

Saitoh, E

Isemura, S

Chiba, A

Oka, S

Odani, S

机构：

[1] Nippon Dent Univ, Sch Dent Niigata, Dept Biochem, Niigata 9518580, Japan

[2] Niigata Univ, Nippon Dent Coll, Niigata 9518580, Japan

[3] Nippon Dent Univ, Sch Dent Niigata, Dept Biol, Niigata 9518580, Japan

[4] Niigata Univ, Fac Sci, Dept Biol, Niigata 9502181, Japan

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 2005年 / 141卷 / 01期

基金：

日本学术振兴会;

关键词：

AJL-2; defensive factor at the eel body surface; cysteine protease inhibitor; cysteine proteases of papain family; Eel-CPI-I; eel skin lectin; nonspecific defense mechanism; skin extract of the eel; Japanese eel Anguilla japonica;

D O I：

10.1016/j.cbpc.2005.02.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel cysteine protease inhibitor (Eel-CPI-1) was isolated from the epidermis of the eel. Eel-CPI-1 was shown to bind strongly to both lactose- and carboxymethylated papain-affinity gels. Its molecular mass under reducing condition was determined to be 18 kDa by SDS-polyacrylamide gel electrophoresis but approximately 30.5 kDa under non-reducing-conditions. Eel-CPI-1 inhibited papain (K-i= 18 nM) and ficin (K-i = 120 nM) competitively. Combined with the data on amino acid and sequence analysis, Eel-CPI-1 is identical to the eel lectin, AJL-2. This is the first report describing a cysteine protease inhibitor with lectin activity. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：103 / 109

页数：7

共 31 条

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