Co-expression of CD147 (EMMPRIN), CD44v3-10, MDR1 and monocarboxylate transporters is associated with prostate cancer drug resistance and progression

被引：99
作者：
Hao, J. [1 ,2 ]
Chen, H. [1 ,2 ]
Madigan, M. C. [3 ]
Cozzi, P. J. [1 ,4 ]
Beretov, J. [1 ,2 ,5 ]
Xiao, W. [1 ,2 ]
Delprado, W. J. [6 ]
Russell, P. J. [1 ,7 ,8 ]
Li, Y. [1 ,2 ]
机构：
[1] UNSW, Fac Med, Kensington, NSW 2052, Australia
[2] St George Hosp, Canc Care Ctr, Kogarah, NSW 2217, Australia
[3] UNSW, Sch Optometry & Vis Sci, Kensington, NSW 2052, Australia
[4] St George Hosp, Dept Surg, Kogarah, NSW 2217, Australia
[5] St George Hosp, Dept Anat Pathol, Kogarah, NSW 2217, Australia
[6] Douglass Hanly Moir Pathol, N Ryde, NSW 2113, Australia
[7] UNSW, Prince Wales Clin Sch, Oncol Res Ctr, Randwick, NSW 2031, Australia
[8] Queensland Univ Technol, Inst Hlth & Biomed Innovat, Australian Prostate Canc Res Ctr Queensland, Kelvin Grove, Qld 1059, Australia
来源：
BRITISH JOURNAL OF CANCER | 2010年 / 103卷 / 07期
关键词：
prostate cancer; CD147; CD44v3-10; monocarboxylate transporters; multidrug resistance; metastasis; MATRIX METALLOPROTEINASE INDUCER; MULTIDRUG-RESISTANCE; HYALURONIC-ACID; P-GLYCOPROTEIN; CARCINOMA CELLS; CD44; ISOFORM; TUMOR-CELLS; EXPRESSION; INVASION; LACTATE;
D O I：
10.1038/sj.bjc.6605839
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
BACKGROUND: The aim of this study is to seek an association between markers of metastatic potential, drug resistance-related protein and monocarboxylate transporters in prostate cancer (CaP). METHODS: We evaluated the expression of invasive markers (CD147, CD44v3-10), drug-resistance protein (MDR1) and monocarboxylate transporters (MCT1 and MCT4) in CaP metastatic cell lines and CaP tissue microarrays (n = 140) by immunostaining. The co-expression of CD147 and CD44v3-10 with that of MDR1, MCT1 and MCT4 in CaP cell lines was evaluated using confocal microscopy. The relationship between the expression of CD147 and CD44v3-10 and the sensitivity (IC(50)) to docetaxel in CaP cell lines was assessed using MTT assay. The relationship between expression of CD44v3-10, MDR1 and MCT4 and various clinicopathological CaP progression parameters was examined. RESULTS: CD147 and CD44v3-10 were co-expressed with MDR1, MCT1 and MCT4 in primary and metastatic CaP cells. Both CD147 and CD44v3-10 expression levels were inversely related to docetaxel sensitivity (IC50) in metastatic CaP cell lines. Overexpression of CD44v3-10, MDR1 and MCT4 was found in most primary CaP tissues, and was significantly associated with CaP progression. CONCLUSIONS: Our results suggest that the overexpression of CD147, CD44v3-10, MDR1 and MCT4 is associated with CaP progression. Expression of both CD147 and CD44v3-10 is correlated with drug resistance during CaP metastasis and could be a useful potential therapeutic target in advanced disease. British Journal of Cancer (2010) 103, 1008-1018. doi:10.1038/sj.bjc.6605839 www.bjcancer.com Published online 24 August 2010 (C) 2010 Cancer Research UK
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收藏
页码：1008 / 1018
页数：11
相关论文
共 54 条
[1]   Hyperpolarized 13C Lactate, Pyruvate, and Alanine: Noninvasive Biomarkers for Prostate Cancer Detection and Grading [J].
Albers, Mark J. ;
Bok, Robert ;
Chen, Albert P. ;
Cunningham, Charles H. ;
Zierhut, Matt L. ;
Zhang, Vickie Yi ;
Kohler, Susan J. ;
Tropp, James ;
Hurd, Ralph E. ;
Yen, Yi-Fen ;
Nelson, Sarah J. ;
Vigneron, Daniel B. ;
Kurhanewicz, John .
CANCER RESEARCH, 2008, 68 (20) :8607-8615
[2]   Hyaluronic acid-paclitaxel: Antitumor efficacy against CD44(+) human ovarian carcinoma xenografts [J].
Auzenne, Edmond ;
Ghosh, Sukhen C. ;
Khodadadian, Mojgan ;
Rivera, Belinda ;
Farquhar, David ;
Price, Roger E. ;
Ravoori, Murali ;
Kundra, Vikas ;
Freedman, Ralph S. ;
Klostergaard, Jim .
NEOPLASIA, 2007, 9 (06) :479-486
[3]  
Chen CT, 1998, CANCER RES, V58, P2777
[4]   The multidrug transporter P-glycoprotein: A mediator of melanoma invasion? [J].
Colone, Marisa ;
Calcabrini, Annarica ;
Toccacieli, Laura ;
Bozzuto, Giuseppina ;
Stringaro, Annarita ;
Gentile, Massimo ;
Cianfriglia, Maurizio ;
Ciervo, Alessandra ;
Caraglia, Michele ;
Budillon, Alfredo ;
Meo, Giuseppina ;
Arancia, Giuseppe ;
Molinari, Agnese .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2008, 128 (04) :957-971
[5]   Evaluation of urokinase plasminogen activator and its receptor in different grades of human prostate cancer [J].
Cozzi, Paul J. ;
Wang, Jian ;
Delprado, Warick ;
Madigan, Michele C. ;
Fairy, Stephen ;
Russell, Pamela J. ;
Li, Yong .
HUMAN PATHOLOGY, 2006, 37 (11) :1442-1451
[6]  
De Marzo AM, 1998, PROSTATE, V34, P162
[7]   Anti-EMMPRIN Monoclonal Antibody as a Novel Agent for Therapy of Head and Neck Cancer [J].
Dean, Nichole R. ;
Newman, J. Robert ;
Helman, Emily E. ;
Zhang, Wenyue ;
Safavy, Seena ;
Weeks, D. M. ;
Cunningham, Mark ;
Snyder, Linda A. ;
Tang, Yi ;
Yan, Li ;
McNally, Lacey R. ;
Buchsbaum, Donald J. ;
Rosenthal, Eben L. .
CLINICAL CANCER RESEARCH, 2009, 15 (12) :4058-4065
[8]   Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia [J].
Deora, AA ;
Philp, N ;
Hu, J ;
Bok, D ;
Rodriguez-Boulan, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) :16245-16250
[9]   CpG hypermethylation of MDR1 gene contributes to the pathogenesis and progression of human prostate cancer [J].
Enokida, H ;
Shiina, H ;
Igawa, M ;
Ogishima, T ;
Kawakami, T ;
Bassett, WW ;
Anast, JW ;
Li, LC ;
Urakami, S ;
Terashima, M ;
Verma, M ;
Kawahara, M ;
Nakagawa, M ;
Kane, CJ ;
Carroll, PR ;
Dahiya, R .
CANCER RESEARCH, 2004, 64 (17) :5956-5962
[10]   Monocarboxylate transporter 4 regulates maturation and trafficking of CD147 to the plasma membrane in the metastatic breast cancer cell line MDA-MB-231 [J].
Gallagher, Shannon M. ;
Castorino, John J. ;
Wang, Dian ;
Philp, Nancy J. .
CANCER RESEARCH, 2007, 67 (09) :4182-4189
123456