The roles of different Bacteroides fragilis strains in protecting against DSS-induced ulcerative colitis and related functional genes

被引：4
作者：
Wang, Chen [1 ,2 ]
Li, Sijia [1 ,2 ]
Hong, Kan [3 ]
Yu, Leilei [1 ,2 ]
Tian, Fengwei [1 ,2 ]
Zhao, Jianxin [1 ,2 ]
Zhang, Hao [1 ,2 ,4 ,5 ,6 ]
Chen, Wei [1 ,2 ,4 ]
Zhai, Qixiao [1 ,2 ]
机构：
[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Jiangsu, Peoples R China
[3] Nanjing Med Univ, Wuxi Peoples Hosp, Wuxi 214023, Jiangsu, Peoples R China
[4] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Jiangsu, Peoples R China
[5] Wuxi Translat Med Res Ctr, Wuxi, Jiangsu, Peoples R China
[6] Jiangsu Translat Med Res Inst, Wuxi Branch, Wuxi 214122, Jiangsu, Peoples R China
来源：
FOOD & FUNCTION | 2021年 / 12卷 / 18期
基金：
中国国家自然科学基金;
关键词：
COMMENSAL; GUT; COLONIZATION; PERFORMANCE; MICROBIOTA; INFECTION; DISEASE; MICE;
D O I：
10.1039/d1fo00875g
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The role of supplementation with different Bacteroides fragilis (B. fragilis) strains in alleviating ulcerative colitis (UC) is unclear due to the controversial results from animal experiments. In this study, three B. fragilis strains were evaluated for their ability to alleviate dextran sulfate sodium (DSS)-induced UC in C57BL/6J mice. We analyzed the anti-inflammatory effects of different B. fragilis strains and the changes they caused in the intestinal microbiota composition, intestinal epithelial permeability, cytokine concentrations, protein expression of nuclear factor kappa-B (NF-kappa B) and the underlying specific genes. The results showed that when orally administered, the different B. fragilis strains exerted different effects on the assessed parameters of the mice. The results of real-time quantitative polymerase chain reaction and immunofluorescence staining showed that the supplementation of B. fragilis FSHCM14E1, but not FJSWX11BF, enhanced the expression of the tight-junction proteins ZO-1, occludin and claudin-1. Western blot analysis showed that the anti-inflammatory effects of B. fragilis FSHCM14E1 were related to the NF-kappa B pathway. Genomic analysis suggested that the anti-inflammatory effects of FSHCM14E1 may be mediated through specific genes associated with defense mechanisms and the secretion of SCFAs. Overall, this study indicates the therapeutic potential of B. fragilis FSHCM14E1 for the prevention of UC.
引用
收藏
页码：8300 / 8313
页数：14
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