Microarray-based identification of tomato microRNAs and time course analysis of their response to Cucumber mosaic virus infection

被引:20
|
作者
Lang, Qiu-lei [1 ,2 ]
Zhou, Xiao-chuan [3 ]
Zhang, Xiao-lin [3 ]
Drabek, Rafal [4 ]
Zuo, Zhi-xiang [3 ]
Ren, Yong-liang [3 ]
Li, Tong-bin [3 ]
Chen, Ji-shuang [1 ,2 ]
Gao, Xiao-lian [3 ,4 ]
机构
[1] Zhejiang Univ, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China
[2] Zhejiang Sci Tech Univ, Inst Bioengn, Hangzhou 310018, Zhejiang, Peoples R China
[3] LC Sci, Houston, TX 77054 USA
[4] Univ Houston, Dept Biol & Biochem, Houston, TX 77004 USA
来源
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B | 2011年 / 12卷 / 02期
基金
中国国家自然科学基金;
关键词
Solanum lycopersicum; Plant miRNA; Cucumber mosaic virus; miRNA array; miRNA response; SMALL RNAS; CONSERVED MICRORNAS; FRUIT; SUPPRESSOR; TARGETS; GENES;
D O I
10.1631/jzus.B1000278
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A large number of plant microRNAs (miRNAs) are now documented in the miRBase, among which only 30 are for Solanum lycopersicum (tomato). Clearly, there is a far-reaching need to identify and profile the expression of miRNAs in this important crop under various physiological and pathological conditions. In this study, we used an in situ synthesized custom microarray of plant miRNAs to examine the expression and temporal presence of miRNAs in the leaves of tomato plants infected with Cucumber mosaic virus (CMV). Following computational sequence homology search and hairpin structure prediction, we identified three novel tomato miRNA precursor genes. Our results also show that, in accordance with the phenotype of the developing leaves, the tomato miRNAs are differentially expressed at different stages of plant development and that CMV infection can induce or suppress the expression of miRNAs as well as up-regulate some star miRNAs (miRNA*s) which are normally present at much lower levels. The results indicate that developmental anomalies elicited by virus infection may be caused by more complex biological processes.
引用
收藏
页码:116 / 125
页数:10
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