Metabolic Engineering of Lysine Producing Corynebacterium glutamicum Strains

被引：3

作者：

Andriiash, G. S. ^{[1
]}

Sekan, O. S. ^{[1
,2
]}

Tigunova, O. O. ^{[1
]}

Blume, Ya. B. ^{[1
]}

Shulga, S. M. ^{[1
]}

机构：

[1] Natl Acad Sci Ukraine, Inst Food Biotechnol & Genom, UA-04123 Kiev, Ukraine

[2] Lulea Univ Technol, Div Wood Sci & Engn, Dept Engn Sci & Math, S-93187 Skelleftea, Sweden

来源：

CYTOLOGY AND GENETICS | 2020年 / 54卷 / 02期

关键词：

Keywords; Corynebacterium glutamicum; producer strain; microbiological synthesis; lysine; metabolic engineering; ACID-PRODUCING BACTERIUM; AMINO-ACIDS; PHOSPHOTRANSFERASE SYSTEM; EXPRESSION SYSTEM; GENE-EXPRESSION; RNA-POLYMERASE; GLUTAMATE; FLUX; PATHWAYS; GLUCOSE;

D O I：

10.3103/S0095452720020024

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The review is devoted to the analysis of the current achievements of Corynebacterium glutamicum metabolic engineering for the production of lysine. Key genes of lysine biosynthesis in C. glutamicum and ways of creating new genetically modified strains are considered. The role of different plasmids, vector cassettes, and promoter types for the regulation of gene expression in C. glutamicum is described. Information is provided on the use of carbon-containing substrates (hexose, pentose, lactic acid, mannitol) for the production of lysine. Possibilities of using CRISPR technology in genetic engineering of C. glutamicum are considered. Genetic changes in C. glutamicum allowed the use of alternative substrates and contributed to the increase of lysine accumulation in the culture fluid. The data that may be used for the creation of new lysine overproduction strains are summarized.

引用

页码：137 / 146

页数：10

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