The dichloromethane fraction from Mahonia bealei (Fort.) Carr. leaves exerts an anti-inflammatory effect both in vitro and in vivo

被引:27
作者
Hu, Weicheng [1 ]
Wu, Lei [1 ,2 ]
Qiang, Qian [1 ]
Ji, Lilian [1 ]
Wang, Xinfeng [1 ]
Luo, Haiqing [1 ]
Wu, Haifeng [3 ,4 ]
Jiang, Yunyao [5 ]
Wang, Gongcheng [6 ]
Shen, Ting [1 ]
机构
[1] Huaiyin Normal Univ, Jiangsu Collaborat Innovat Ctr Reg Modern Agr & E, Jiangsu Key Lab Ecoagr Biotechnol Hongze Lake, Huaian 223300, Peoples R China
[2] Tianjin Univ Sci & Technol, Tianjin Key Lab Pulp & Paper, Tianjin 300457, Peoples R China
[3] Peking Union Med Coll, Inst Med Plant Dev, Key Lab Bioact Subst & Resources Utilizat Chinese, Minist Educ, Beijing 100193, Peoples R China
[4] Chinese Acad Med Sci, Beijing 100193, Peoples R China
[5] China Acad Chinese Med Sci, Xiyuan Hosp, Beijing 100091, Peoples R China
[6] Nanjing Med Univ, Huaian Peoples Hosp 1, Dept Urol, 6 Beijing West Rd, Huaian 223300, Peoples R China
关键词
Mahonia bealei leaf; Lipopolysaccharide; Acute lung injury; Anti-inflammatory; NF-kappa B; NF-KAPPA-B; ACUTE LUNG INJURY; RAW; 264.7; CELLS; NITRIC-OXIDE PRODUCTION; PHOSPHATIDYLINOSITOL; 3-KINASE; INFLAMMATORY MEDIATORS; WATER EXTRACT; ACTIVATION; MACROPHAGES; INHIBITION;
D O I
10.1016/j.jep.2016.05.013
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Ethnopharmacological relevance: Mahonia bealei has a long history of medical use in traditional Chinese medicine for the treatment of inflammatory-associated diseases. Despite numerous phytochemical and pharmacological studies, there is a lack of systematic studies to understand the cellular and molecular mechanisms of the anti-inflammatory activity of this plant. Aim of study: This study aimed to evaluate the anti-inflammatory activity of the dichloromethane fraction from M. bealei leaves (MBL-CH). Materials and methods: RAW 264.7 cells were pretreated with different concentrations of MBL-CH for 30 min prior to treatment with 1 mu g/ml of lipopolysaccharide (LPS). The nuclear factor kappa B (NF-kappa B) pathway and subsequent production of inflammatory mediators, such as nitric oxide (NO), prostaglandin E-2 (PGE(2)), and tumour necrosis factor (TNF)-alpha were investigated. Furthermore, the in vivo mouse model of LPS-induced acute lung injury (ALI) was employed to study the anti-inflammatory effects of MBL-CH. Results: Pre-treatment with MBL-CH significantly inhibited the LPS-stimulated secretion of NO, PGE(2), and TNF-alpha into the culture medium, as well as the mRNA levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and TNF-alpha, which were associated with a reduction in the phosphorylation of I kappa B alpha, Akt, and PI3K and inhibition of the transcriptional activity of NF-kappa B. Furthermore, in vivo experiments revealed that MBL-CH attenuated LPS-stimulated lung inflammation in mice. Conclusion: Taken together, our findings indicate that MBL-CH attenuates LPS-stimulated inflammatory responses in macrophages by blocking NF-kappa B activation through interference with activation of the PI3K/Akt pathway, providing scientific evidence that the plant can be employed in traditional remedies. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:134 / 143
页数:10
相关论文
共 60 条
  • [1] Toll-like receptors in the induction of the innate immune response
    Aderem, A
    Ulevitch, RJ
    [J]. NATURE, 2000, 406 (6797) : 782 - 787
  • [2] Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis
    Adya, Raghu
    Tan, Bee K.
    Punn, Anu
    Chen, Jing
    Randeva, Harpal S.
    [J]. CARDIOVASCULAR RESEARCH, 2008, 78 (02) : 356 - 365
  • [3] Saffron: A Potential Candidate for a Novel Anticancer Drug Against Hepatocellular Carcinoma
    Amin, Amr
    Hamza, Alaaeldin A.
    Bajbouj, Khuloud
    Ashraf, S. Salman
    Daoud, Sayel
    [J]. HEPATOLOGY, 2011, 54 (03) : 857 - 867
  • [4] [Anonymous], 2001, FLORA CHINA
  • [5] Role of NF-κB and Akt/PI3K in the resistance of pancreatic carcinoma cell lines against gemcitabine-induced cell death
    Arlt, A
    Gehrz, A
    Müerköster, S
    Vorndamm, J
    Kruse, ML
    Fölsch, UR
    Schäfer, H
    [J]. ONCOGENE, 2003, 22 (21) : 3243 - 3251
  • [6] Involvement of reactive oxygen species in Toll-like receptor 4-dependent activation of NF-κB
    Asehnoune, K
    Strassheim, D
    Mitra, S
    Kim, JY
    Abraham, E
    [J]. JOURNAL OF IMMUNOLOGY, 2004, 172 (04) : 2522 - 2529
  • [7] The anti-inflammatory activities of Ainsliaea fragrans Champ. extract and its components in lipopolysaccharide-stimulated RAW264.7 macrophages through inhibition of NF-κB pathway
    Chen, Xin
    Miao, Jingshan
    Wang, Hao
    Zhao, Fang
    Hu, Jie
    Gao, Peng
    Wang, Yue
    Zhang, Luyong
    Yan, Ming
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2015, 170 : 72 - 80
  • [8] Acute Lung Injury: Apoptosis and Signaling Mechanisms
    Chopra, Mani
    Reuben, Jayne S.
    Sharma, Avadhesh C.
    [J]. EXPERIMENTAL BIOLOGY AND MEDICINE, 2009, 234 (04) : 361 - 371
  • [9] Endothelial nitric oxide synthase: the Cinderella of inflammation?
    Cirino, G
    Fiorucci, S
    Sessa, WC
    [J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 2003, 24 (02) : 91 - 95
  • [10] Inflammation: the link between insulin resistance, obesity and diabetes
    Dandona, P
    Aljada, A
    Bandyopadhyay, A
    [J]. TRENDS IN IMMUNOLOGY, 2004, 25 (01) : 4 - 7