Apoptotic Effect of Nigella sativa on Human Lymphoma U937 Cells

被引：11
|
作者：
Arslan, Belkis Atasever [1 ]
Isik, Fatma Busra [1 ]
Gur, Hazal [2 ]
Ozen, Fatih [2 ]
Catal, Tunc [1 ]
机构：
[1] Uskudar Univ, Fac Engn & Nat Sci, Dept Mol Biol & Genet, Istanbul, Turkey
[2] Uskudar Univ, Fac Engn & Nat Sci, Dept Bioengn, Istanbul, Turkey
来源：
PHARMACOGNOSY MAGAZINE | 2017年 / 13卷 / 51期
关键词：
Apoptosis; caspase-3; human lymphoma U937 cells; Nigella sativa; p53; thymoquinone; METHANOLIC EXTRACT; ESSENTIAL OILS; THYMOQUINONE; BAD; P53; MITOCHONDRIA; AGENTS; ROLES; PARTS; GENE;
D O I：
10.4103/pm.pm_93_17
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Objective: Nigella sativa is from botanical Ranunculaceae family and commonly known as black seed. Apoptotic effect of N. sativa and its apoptotic signaling pathways on U937 lymphoma cells are unknown. Materials and Methods: In this study, we investigated selective cytotoxic and apoptotic effects of N. sativa extract and its apoptotic mechanisms on U937 cells. In addition, we also studied selective cytotoxic activity of thymoquinone that is the most active essential oil of N. sativa. Results: Our results showed that N. sativa extract has selective cytotoxicity and apoptotic effects on U937 cells but not ECV304 control cells. However, thymoquinone had no significant cytotoxicity against on both cells. N. sativa extract increased significantly caspase-3, BAD, and p53 gene expressions in U937 cells. Conclusions: N. sativa may have anticancer drug potential and trigger p53-induced apoptosis in U937 lymphoma cells.
引用
收藏
页码：S628 / S632
页数：5
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