Apoptotic Effect of Nigella sativa on Human Lymphoma U937 Cells

被引：14

作者：

Arslan, Belkis Atasever ^{[1
]}

Isik, Fatma Busra ^{[1
]}

Gur, Hazal ^{[2
]}

Ozen, Fatih ^{[2
]}

Catal, Tunc ^{[1
]}

机构：

[1] Uskudar Univ, Fac Engn & Nat Sci, Dept Mol Biol & Genet, Istanbul, Turkey

[2] Uskudar Univ, Fac Engn & Nat Sci, Dept Bioengn, Istanbul, Turkey

来源：

PHARMACOGNOSY MAGAZINE | 2017年 / 13卷 / 51期

关键词：

Apoptosis; caspase-3; human lymphoma U937 cells; Nigella sativa; p53; thymoquinone; METHANOLIC EXTRACT; ESSENTIAL OILS; THYMOQUINONE; BAD; P53; MITOCHONDRIA; AGENTS; ROLES; PARTS; GENE;

D O I：

10.4103/pm.pm_93_17

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Objective: Nigella sativa is from botanical Ranunculaceae family and commonly known as black seed. Apoptotic effect of N. sativa and its apoptotic signaling pathways on U937 lymphoma cells are unknown. Materials and Methods: In this study, we investigated selective cytotoxic and apoptotic effects of N. sativa extract and its apoptotic mechanisms on U937 cells. In addition, we also studied selective cytotoxic activity of thymoquinone that is the most active essential oil of N. sativa. Results: Our results showed that N. sativa extract has selective cytotoxicity and apoptotic effects on U937 cells but not ECV304 control cells. However, thymoquinone had no significant cytotoxicity against on both cells. N. sativa extract increased significantly caspase-3, BAD, and p53 gene expressions in U937 cells. Conclusions: N. sativa may have anticancer drug potential and trigger p53-induced apoptosis in U937 lymphoma cells.

引用

页码：S628 / S632

页数：5

共 31 条

[1] Can methanolic extract of Nigella sativa seed affect glyco-regulatory enzymes in experimental hepatocellular carcinoma? [J].

Abdel-Hamid, N. M. ;

Abdel-Ghany, M. I. ;

Nazmy, M. H. ;

Amgad, S. W. .

ENVIRONMENTAL HEALTH AND PREVENTIVE MEDICINE, 2013, 18 (01) :49-56

[2]

Abukhader Majed M, 2013, Pharmacogn Rev, V7, P117, DOI 10.4103/0973-7847.120509

[3]

Al-Amri A. M., 2009, Shiraz E Medical Journal, V10, P107

[4] Roles of p53 and Caspases in Induction of Apoptosis in MCF-7 Breast Cancer Cells Treated with a Methanolic Extract of Nigella Sativa Seeds [J].

Alhazmi, Mohammed I. ;

Hasan, Tarique N. ;

Shafi, Gowhar ;

Al-Assaf, Abdullah H. ;

Alfawaz, Mohammed A. ;

Alshatwi, Ali A. .

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION, 2014, 15 (22) :9655-9660

[5] CHEMICAL-COMPOSITION AND MICROFLORA OF BLACK CUMIN (NIGELLA-SATIVA L) SEEDS GROWING IN SAUDI-ARABIA [J].

ALJASSIR, MS .

FOOD CHEMISTRY, 1992, 45 (04) :239-242

[6] Radioprotective effects of black seed (Nigella sativa) oil against hemopoietic damage and immunosuppression in gamma-irradiated rats [J].

Assayed, Mohamed E. .

IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY, 2010, 32 (02) :284-296

[7] Screening of new antileukemic agents from essential oils of algae extracts and computational modeling of their interactions with intracellular signaling nodes [J].

Ataseyer-Arslan, Belkis ;

Yilancioglu, Kaan ;

Kalkan, Zeynep ;

Timucin, Ahmet Can ;

Gur, Hazal ;

Isik, Fatma Busra ;

Deniz, Emre ;

Erman, Batu ;

Cetiner, Selim .

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES, 2016, 83 :120-131

[8] Cytotoxic effect of extract from Dunaliella salina against SH-SY5Y neuroblastoma cells [J].

Ataseyer-Arslan, Belkis ;

Yilancioglu, Kaan ;

Bekaroglu, Maide G. ;

Taskin, Emre ;

Altinoz, Eyup ;

Cetiner, Selim .

GENERAL PHYSIOLOGY AND BIOPHYSICS, 2015, 34 (02) :201-207

[9] NIGELLIDINE - A NEW INDAZOLE ALKALOID FROM THE SEEDS OF NIGELLA-SATIVA [J].

ATTAURRAHMAN ;

MALIK, S ;

HASAN, SS ;

CHOUDHARY, MI ;

NI, CZ ;

CLARDY, J .

TETRAHEDRON LETTERS, 1995, 36 (12) :1993-1996

[10] Review on Molecular and Therapeutic Potential of Thymoquinone in Cancer [J].

Banerjee, Sanjeev ;

Padhye, Subhash ;

Azmi, Asfar ;

Wang, Zhiwei ;

Philip, Philip A. ;

Kucuk, Omer ;

Sarkar, Fazlul H. ;

Mohammad, Ramzi M. .

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2010, 62 (07) :938-946

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