Carthamus tinctorius L. prevents LPS-induced TNFα signaling activation and cell apoptosis through JNK1/2-NFκB pathway inhibition in H9c2 cardiomyoblast cells

被引:71
作者
Tien, Yun-Chen [2 ]
Lin, Jing-Ying [3 ]
Lai, Chao-Hung [4 ]
Kuo, Chia-Hua [5 ]
Lin, Wen-Yuan [6 ]
Tsai, Chang-Hai [7 ]
Tsai, Fuu-Jen [8 ]
Cheng, Yi-Chang [9 ]
Peng, Wen-Huang [2 ]
Huang, Chih-Yang [1 ,7 ,8 ]
机构
[1] China Med Univ, Grad Inst Basic Med Sci, Taichung 404, Taiwan
[2] China Med Univ, Grad Inst Chinese Pharmaceut Sci, Taichung 404, Taiwan
[3] Cent Taiwan Univ Sci & Technol, Dept Med Imaging & Radiol Sci, Taichung, Taiwan
[4] Armed Force Taichung Gen Hosp, Div Cardiol, Taichung, Taiwan
[5] Taipei Phys Educ Coll, Lab Exercise Biochem, Taipei, Taiwan
[6] China Med Univ & Hosp, Dept Family Med, Taichung, Taiwan
[7] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung, Taiwan
[8] China Med Univ, Dept Chinese Med, Taichung 404, Taiwan
[9] Taichung Vet Gen Hosp, Emergency Dept, Taichung, Taiwan
关键词
H9c2 cardiomyoblast apoptosis; Lipopolysaccharide; Carthamus tinctorius L; JNK; TNF alpha; NF-KAPPA-B; CHRONIC HEART-FAILURE; TUMOR-NECROSIS-FACTOR; MYOCYTE APOPTOSIS; BASIC MECHANISMS; RECEPTOR; ENDOTOXIN; CONTRACTILITY; MITOCHONDRIA; EXPRESSION;
D O I
10.1016/j.jep.2010.05.038
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Aim of the study: Severe and potentially fatal hypotension and cardiac contractile dysfunction are common symptoms in patients with sepsis. In our previous study, we found that estradiol and estrogen-receptor a have cardio-protective effects in myocardial cells exposed to LPS. Estradiol supplementation has been shown to induce breast and cervical cancers. Flos Carthami, the flower of Carthamus tinctorius L (Compositae) is an important traditional Chinese medicine used for the treatment of heart disease and inflammation, and therefore might be a potential alternative to Estradiol in the prevention of heart damage. This study investigated the effect of Flos Carthami (FCEtOH) ethanolic extract on LPS-induced apoptosis in H9c2 cardiomyoblast cells. Materials and methods: H9c2 cells induced apoptosis with LPS administration (1 mu g/mL). H9c2 cells were divided into five groups: Control, LPS (1 mu g/mL), and three FCEtOH (31.25, 62.5,and 125 mu g/mL). We detected apoptosis using MTT, LDH, TUNEL assay. JC-1 staining and Western blot were used to detect pro-apoptosis proteins, anti-apoptosis proteins, MAPK proteins (JNK, ERK, and P38), and NF kappa B expression. Results: FCEtOH (62.5 mu g/mL) inhibited LPS-induced apoptosis by suppressing JNK1/2 activity, which resulted in the reduction of both I kappa B degradation and NF kappa B activation. In addition, FCEtOH led to the activation of anti-apoptotic proteins, Bcl-2 and Bcl-xL, the stabilization of the mitochondria membrane and the down-regulation of extrinsic and intrinsic pro-apoptotic proteins, such as TNF alpha, active caspase-8, t-Bid, Bax, active caspases-9, and -3. Conclusions: Carthamus tinctorius L possesses the ability to suppress JNK activity and inhibit LPS-induced TNF alpha activation and apoptosis in H9c2 cardiomyoblast cells. Carthamus tinctorius L could potentially serve as a cardio-protective agent against LPS-induced apoptosis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:505 / 513
页数:9
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