Strain differences regarding susceptibility to ursolic acid-induced interleukin-1β release in murine macrophages

Interleukin (IL)-1 beta is a proinflammatory cytokine responsible for the onset of a broad range of diseases, such as inflammatory bowel disease and rheumatoid arthritis. We have recently found that aggregated ursolic acid (UA), a triterpene carboxylic acid, is recognized by CD36 for generating reactive oxygen species (ROS) via NADPH oxidase (NOX) activation, thereby releasing IL-1 beta protein from murine peritoneal macrophages (pM phi) in female ICR mice. In the present study, we investigated the ability of UA for inducing IL-1 beta production in pM phi from 4 different strains of female mice (C57BL/6J, C3H/He, DDY, and ICR), as well as an established macrophage line (RAW264.7 cells). The levels of IL-1 beta released from UA-treated pM phi of C57BL/6J and DDY mice were significantly lower than from those of ICR mice, whereas IL-1 beta was not released from the pM phi of C3H/He mice or RAW264.7 cells. Of paramount importance, CD36 as well as the NOX components gp91(phox) and p47(phox) (C3H/He mice) and gp91(phox) (RAW264.7 cells) were scarcely detected. In addition, the different susceptibilities to UA-induced IL-1 beta release were suggested to be correlated with the amount of superoxide anion (O-2(-)) generated from the 5 different types of M phi. Notably, intracellular, but not extracellular, O-2(-) generation was indicated to play a major role in UA-induced IL-1 beta release. Together, our results indicate that the UA-incluced IL-1 beta release was strain-dependent, and the expression status of CD36 and gp91(phox) is strongly associated with inducibility. (C) 2008 Elsevier Inc. All rights reserved.