Internal pH indicators for biomolecular NMR

被引：70

作者：

Baryshnikova, Olga K. ^{[1
]}

Williams, Thomas C. ^{[1
]}

Sykes, Brian D. ^{[1
]}

机构：

[1] Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada

来源：

JOURNAL OF BIOMOLECULAR NMR | 2008年 / 41卷 / 01期

关键词：

internal indicator; biomolecular NMR; pH;

D O I：

10.1007/s10858-008-9234-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a non-invasive technique for determining pH in biomolecular NMR sample using buffer components (formate, tris, piperazine, and imidazole) as internal pH indicators, whose H-1 NMR chemical shifts are sensitive to pH in a range from 2.5 to 9.8. This method is suitable for a wide range of applications where samples are handled intensively during NMR titrations or in high throughput analysis in structural genomics or metabolomics.

引用

页码：5 / 7

页数：3

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