Neutrophil Extracellular Traps Are Pathogenic in Ventilator-Induced Lung Injury and Partially Dependent on TLR4

被引：37
作者：
Li, Haosi [1 ]
Pan, Pinhua [1 ]
Su, Xiaoli [1 ]
Liu, Shuai [1 ]
Zhang, Lemeng [2 ,3 ]
Wu, Dongdong [1 ]
Li, Haitao [1 ]
Dai, Minhui [1 ]
Li, Yi [1 ]
Hu, Chengping [1 ]
Chen, Jie [1 ]
机构：
[1] Cent South Univ, Xiangya Hosp, Dept Pulm & Crit Care Med, Changsha 410008, Hunan, Peoples R China
[2] Cent South Univ, Hunan Canc Hosp, Dept Thorac Med, Changsha 410013, Hunan, Peoples R China
[3] Cent South Univ, Xiangya Sch Med, Affiliated Canc Hosp, Changsha 410013, Hunan, Peoples R China
来源：
BIOMED RESEARCH INTERNATIONAL | 2017年 / 2017卷
基金：
中国国家自然科学基金;
关键词：
TIDAL-VOLUME VENTILATION; MECHANICAL VENTILATION; RECEPTOR; ACTIVATION; CONTRIBUTES; EXPRESSION; STRATEGIES; MEDIATORS; HISTONES; CAPTURE;
D O I：
10.1155/2017/8272504
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The pathogenesis of ventilator-induced lung injury (VILI) is associated with neutrophils. Neutrophils release neutrophil extracellular traps (NETs), which are composed of DNA and granular proteins. However, the role of NETs in VILI remains incompletely understood. Normal saline and deoxyribonuclease (DNase) were used to study the role of NETs in VILI. To further determine the role of Toll-like receptor 4 (TLR4) in NETosis, we evaluated the lung injury and NET formation in TLR4 knockout mice and wild-type mice that were mechanically ventilated. Some measures of lung injury and the NETs markers were significantly increased in the VILI group. DNase treatment markedly reduced NETs markers and lung injury. After high-tidal mechanical ventilation, the NETs markers in the TLR4 KO mice were significantly lower than in the WT mice. These data suggest that NETs are generated in VILI and pathogenic in a mouse model of VILI, and their formation is partially dependent on TLR4.
引用
收藏
页数：13
相关论文
共 49 条
  • [1] Neutrophils and acute lung injury
    Abraham, E
    [J]. CRITICAL CARE MEDICINE, 2003, 31 (04) : S195 - S199
  • [2] Histones from Dying Renal Cells Aggravate Kidney Injury via TLR2 and TLR4
    Allam, Ramanjaneyulu
    Scherbaum, Christina Rebecca
    Darisipudi, Murthy Narayana
    Mulay, Shrikant R.
    Haegele, Holger
    Lichtnekert, Julia
    Hagemann, Jan Henrik
    Rupanagudi, Khader Valli
    Ryu, Mi
    Schwarzenberger, Claudia
    Hohenstein, Bernd
    Hugo, Christian
    Uhl, Bernd
    Reichel, Christoph A.
    Krombach, Fritz
    Monestier, Marc
    Liapis, Helen
    Moreth, Kristin
    Schaefer, Liliana
    Anders, Hans-Joachim
    [J]. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2012, 23 (08): : 1375 - 1388
  • [3] Critical role for CXCR2 and CXCR2 ligands during the pathogenesis of ventilator-induced lung injury
    Belperio, JA
    Keane, MP
    Burdick, MD
    Londhe, V
    Xue, YY
    Li, KW
    Phillips, RJ
    Strieter, RM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2002, 110 (11) : 1703 - 1716
  • [4] DAMPs, PAMPs and alarmins: all we need to know about danger
    Bianchi, Marco E.
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 2007, 81 (01) : 1 - 5
  • [5] The receptor for advanced glycation end products (RAGE) enhances autophagy and neutrophil extracellular traps in pancreatic cancer
    Boone, B. A.
    Orlichenko, L.
    Schapiro, N. E.
    Loughran, P.
    Gianfrate, G. C.
    Ellis, J. T.
    Singhi, A. D.
    Kang, R.
    Tang, D.
    Lotze, M. T.
    Zeh, H. J.
    [J]. CANCER GENE THERAPY, 2015, 22 (06) : 326 - 334
  • [6] Neutrophil extracellular traps kill bacteria
    Brinkmann, V
    Reichard, U
    Goosmann, C
    Fauler, B
    Uhlemann, Y
    Weiss, DS
    Weinrauch, Y
    Zychlinsky, A
    [J]. SCIENCE, 2004, 303 (5663) : 1532 - 1535
  • [7] Beneficial suicide: why neutrophils die to make NETs
    Brinkmann, Volker
    Zychlinsky, Arturo
    [J]. NATURE REVIEWS MICROBIOLOGY, 2007, 5 (08) : 577 - 582
  • [8] Neutrophil extracellular traps: Is immunity the second function of chromatin?
    Brinkmann, Volker
    Zychlinsky, Arturo
    [J]. JOURNAL OF CELL BIOLOGY, 2012, 198 (05) : 773 - 783
  • [9] Platelets induce neutrophil extracellular traps in transfusion-related acute lung injury
    Caudrillier, Axelle
    Kessenbrock, Kai
    Gilliss, Brian M.
    Nguyen, John X.
    Marques, Marisa B.
    Monestier, Marc
    Toy, Pearl
    Werb, Zena
    Looney, Mark R.
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 2012, 122 (07) : 2661 - 2671
  • [10] Mouse Neutrophil Extracellular Traps in Microbial Infections
    Ermert, David
    Urban, Constantin F.
    Laube, Britta
    Goosmann, Christian
    Zychlinsky, Arturo
    Brinkmann, Volker
    [J]. JOURNAL OF INNATE IMMUNITY, 2009, 1 (03) : 181 - 193
12345