Isolation and characterization of microsatellite loci in Sorbus aria (Rosaceae)

被引：8

作者：

Gonzalez-Gonzalez, E. A. ^{[1
]}

Gonzalez-Perez, M. A. ^{[1
]}

Rivero, E. ^{[1
]}

Sosa, P. A. ^{[1
]}

机构：

[1] Univ Las Palmas Gran Canaria, Dept Biol, Las Palmas Gran Canaria 35017, Canary Islands, Spain

来源：

CONSERVATION GENETICS RESOURCES | 2010年 / 2卷

关键词：

Sorbus aria; Microsatellite; Canary Islands; Genetic diversity; Triploid; MALOIDEAE ROSACEAE; DNA;

D O I：

10.1007/s12686-010-9238-x

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Sorbus aria (L) Crantz (Common Whitebeam) is native to Europe, east of the Balkans and in North Africa; it is also present in the Canary Islands. To evaluate the genetic diversity in natural populations of this vulnerable species, nine novel polymorphic microsatellite markers were isolated from enriched libraries. Microsatellite loci were screened in 97 individuals from La Palma (Canary Islands) and Sierra Nevada (Granada, Spain). Examination of the microsatellite profiles shows that S. aria individuals have up to three alleles per locus. The cloned sequences in microsatellite loci confirmed the polyploidy status of the plants. The number of alleles ranged from 5 to 14 per locus. The phenotype diversities across loci (H'(T)) ranging from 0.653 to 0.847.

引用

页码：341 / 343

页数：3

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