Prediction of Cancer-Related piRNAs Based on Network-Based Stratification Analysis

PIWI-interacting RNA (PiRNA) was discovered in 2006 and is expected to become a new biomarker for diagnosis and prognosis of various diseases. The purpose of this study is to explore functions of piRNAs and identify cancer subtypes on the basis of the pattern of transcriptome and somatic mutation data. A total of 285 510 SNPs in piRNAs and genes, which might affect piRNA biogenesis or piRNA targets binding were identified. Significant co-expression networks of piRNAs were then constructed separately for 12 major types of cancer. Finally, mutational matrices were mapped to piRNA network, propagated, and clustered for identification of cancer-related piRNAs and cancer subtypes. Findings showed that subtypes of three types of cancer (COAD, STAD and UCEC), which are significantly associated with survival were identified. Analysis of differentially expressed piRNAs in UCEC subtypes showed that piRNA function is closely related to cancer hallmarks "Enabling Replicative Immortality" and contributes to initiation of cancer.