Vacuolar H+-pyrophosphatase purified from pear fruit

被引：23

作者：

Suzuki, Y ^{[1
]}

Kanayama, Y

Shiratake, K

Yamaki, S

机构：

[1] Nagoya Univ, Grad Sch Bioagr Sci, Hort Sci Lab, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[2] Tohoku Univ, Fac Agr, Lab Hort, Aoba Ku, Sendai, Miyagi 9818555, Japan

来源：

PHYTOCHEMISTRY | 1999年 / 50卷 / 04期

关键词：

Pyrus communis; Rosaceae; pear fruit; enzyme purification; vacuolar H+-translocating inorganic pyrophosphatase; tonoplast;

D O I：

10.1016/S0031-9422(98)00554-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A vacuolar H+-translocating inorganic pyrophosphatase was purified from pear fruit through selective detergent treatments, Superose 6 and Mono Q column chromatography. The specific activity of the purified enzyme was 850 mu mol h(-1) mg protein(-1). The Mr of V-PPase was 66 kDa by SDS-PAGE and the polypeptide cross-reacted with the antiserum against V-PPase of mung bean. The purified V-PPase was stimulated by potassium and inhibited by calcium and N, N'-dicyclohexylcarbodiimide. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：535 / 539

页数：5

共 19 条

[1]

BAIN JOAN M., 1961, AUSTRALIAN JOUR BOT, V9, P99, DOI 10.1071/BT9610099

[2] PURIFICATION AND IMMUNOLOGICAL COMPARISON OF THE TONOPLAST H+-PYROPHOSPHATASE FROM CELLS OF CATHARANTHUS-ROSEUS AND LEAVES FROM MESEMBRYANTHEMUM-CRYSTALLINUM PERFORMING C-3-PHOTOSYNTHESIS AND THE OBLIGATE CAM-PLANT KALANCHOE-DAIGREMONTIANA [J].

BECKER, A ;

CANUT, H ;

LUTTGE, U ;

MAESHIMA, M ;

MARIGO, G ;

RATAJCZAK, R .

JOURNAL OF PLANT PHYSIOLOGY, 1995, 146 (1-2) :88-94

[3] ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS [J].

BENSADOUN, A ;

WEINSTEIN, D .

ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) :241-250

[4] IDENTIFICATION AND PURIFICATION OF SUBSTRATE-BINDING SUBUNIT OF HIGHER-PLANT H+-TRANSLOCATING INORGANIC PYROPHOSPHATASE [J].

BRITTEN, CJ ;

TURNER, JC ;

REA, PA .

FEBS LETTERS, 1989, 256 (1-2) :200-206

[5] TONOPLAST H+-ATPASE OF MATURE PEAR FRUIT [J].

HOSAKA, M ;

KANAYAMA, Y ;

SHIRATAKE, K ;

YAMAKI, S .

PHYTOCHEMISTRY, 1994, 36 (03) :565-567

[6] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[7] Proton pumps of the vacuolar membrane in growing plant cells [J].

Maeshima, M ;

Nakanishi, Y ;

MatsuuraEndo, C ;

Tanaka, Y .

JOURNAL OF PLANT RESEARCH, 1996, 109 (1093) :119-125

[8]

MAESHIMA M, 1989, J BIOL CHEM, V264, P20068

[9]

MAESHIMA M, 1994, PLANT CELL PHYSIOL, V35, P323

[10] H+-TRANSLOCATING INORGANIC PYROPHOSPHATASE OF PLANT VACUOLES - INHIBITION BY CA2+, STABILIZATION BY MG2+ AND IMMUNOLOGICAL COMPARISON WITH OTHER INORGANIC PYROPHOSPHATASES [J].

MAESHIMA, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 196 (01) :11-17

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