Vacuolar H+-pyrophosphatase purified from pear fruit

被引：23
|
作者：
Suzuki, Y [1 ]
Kanayama, Y
Shiratake, K
Yamaki, S
机构：
[1] Nagoya Univ, Grad Sch Bioagr Sci, Hort Sci Lab, Chikusa Ku, Nagoya, Aichi 4648601, Japan
[2] Tohoku Univ, Fac Agr, Lab Hort, Aoba Ku, Sendai, Miyagi 9818555, Japan
来源：
PHYTOCHEMISTRY | 1999年 / 50卷 / 04期
关键词：
Pyrus communis; Rosaceae; pear fruit; enzyme purification; vacuolar H+-translocating inorganic pyrophosphatase; tonoplast;
D O I：
10.1016/S0031-9422(98)00554-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A vacuolar H+-translocating inorganic pyrophosphatase was purified from pear fruit through selective detergent treatments, Superose 6 and Mono Q column chromatography. The specific activity of the purified enzyme was 850 mu mol h(-1) mg protein(-1). The Mr of V-PPase was 66 kDa by SDS-PAGE and the polypeptide cross-reacted with the antiserum against V-PPase of mung bean. The purified V-PPase was stimulated by potassium and inhibited by calcium and N, N'-dicyclohexylcarbodiimide. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：535 / 539
页数：5
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