Detection of human influenza A (H1N1) and B strains with reduced sensitivity to neuraminidase inhibitors

被引：72

作者：

Escuret, Vanessa ^{[1
]}

Frobert, Emilie ^{[1
]}

Bouscambert-Duchamp, Maude ^{[1
]}

Sabatier, Murielle ^{[1
]}

Grog, Isidore ^{[3
]}

Valette, Martine ^{[2
]}

Lina, Bruno ^{[1
,2
]}

Morfin, Florence ^{[1
]}

Ferraris, Olivier ^{[1
]}

机构：

[1] Univ Lyon 1, CNRS, FRE 3011, F-69372 Lyon 08, France

[2] Ctr Biol Est, Hospices Civils Lyon, CNR Virus Influenza France Sud, F-69677 Bron, France

[3] OpenRome, GROG, F-75018 Paris, France

来源：

JOURNAL OF CLINICAL VIROLOGY | 2008年 / 41卷 / 01期

关键词：

influenza; neuraminidase inhibitors; resistance; systematic monitoring;

D O I：

10.1016/j.jcv.2007.10.019

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Background: Neuraminidase inhibitors (NAIs) have been used since 2005 in France. Objective: Influenza viruses isolated in hospital and community cases in winter 2005-2006 were evaluated for their sensitivity to NAIs. Study design: Isolates were tested in duplicate with a fluorescence-based neuraminidase assay. The IC50 for oseltamivir or zanamivir was calculated for each strain. Mean IC50 (+/-S.D.) are expressed in nM. Viruses with IC50 superior or very superior to the upper limit (mean IC50 + 2.5 S.D.) were considered as outliers or resistant viruses, respectively. HA and NA genes for outliers, resistant strains and for a few sensitive strains were sequenced. Results: Out of 225 B isolates, one was found resistant to both oseltamivir and zanamivir with a D197Y mutation in NA and eight isolates were outliers for oseltamivir and/or zanamivir. Out of 151 A (H1N1) isolates, one was found resistant to oseltamivir but sensitive to zanamivir with a H275Y mutation in NA, two isolates were resistant to zanamivir and three isolates were outliers for oseltamivir and/or zanamivir. New mutations were detected in outliers compared to sensitive viruses. Conclusion: Resistant influenza strains to NAIs are circulating at a stable and low level of 1% since the introduction of NAIs in clinical practice. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：25 / 28

页数：4

共 18 条

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