A Proposal for Automated Background Removal of Bio-Raman Data

被引:9
作者
Sugawara, Tatsuro [1 ]
Yang, Qi [1 ]
Nakabayashi, Takakazu [1 ]
Morita, Shin-ichi [2 ]
机构
[1] Tohoku Univ, Grad Sch Pharmaceut Sci, 6-3 Aramaki Aza Aoba, Sendai, Miyagi 9808578, Japan
[2] Tohoku Univ, Grad Sch Sci, 6-3 Aramaki Aza Aoba, Sendai, Miyagi 9808578, Japan
关键词
Raman spectra; cell; background removal; subtraction; quartz substrate; CELL-CYCLE; IN-VIVO; MICROSPECTROSCOPY; SPECTROSCOPY; DYNAMICS; DIFFERENTIATION; DISCRIMINATION; MICROSCOPY; SCATTERING; APOPTOSIS;
D O I
10.2116/analsci.33.1323
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The subtraction of background components from observed spectra is essential in performing multivariable analysis, frequently applied to Raman spectra of cells. The subtraction procedure, however, is manual and time consuming, especially for big data. Here, we propose an automated method for removing background information from measured spectra of cells, exhibiting the theoretical framework and an experimental application.
引用
收藏
页码:1323 / 1325
页数:3
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