Loss-of-function of ASPARTIC PEPTIDASE NODULE-INDUCED 1 (APN1) in Lotus japonicus restricts efficient nitrogen-fixing symbiosis with specific Mesorhizobium loti strains

被引：32

作者：

Yamaya-Ito, Hiroko ^{[1
,2
]}

Shimoda, Yoshikazu ^{[1
]}

Hakoyama, Tsuneo ^{[1
,6
]}

Sato, Shusei ^{[3
,7
]}

Kaneko, Takakazu ^{[3
,8
]}

Hossain, Md Shakhawat ^{[1
,9
,10
]}

Shibata, Satoshi ^{[1
,11
]}

Kawaguchi, Masayoshi ^{[4
]}

Hayashi, Makoto ^{[1
,6
]}

Kouchi, Hiroshi ^{[5
]}

Umehara, Yosuke ^{[1
]}

机构：

[1] Natl Agr & Food Res Org, Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan

[2] Nihon Univ, Coll Bioresource Sci, Fujisawa, Kanagawa 2520800, Japan

[3] Kazusa DNA Res Inst, Kisarazu, Chiba 2920818, Japan

[4] Natl Inst Basic Biol, Okazaki, Aichi 4448585, Japan

[5] Int Christian Univ, Mitaka, Tokyo 1818585, Japan

[6] RIKEN, Ctr Sustainable Resource Sci, Yokohama, Kanagawa 2300045, Japan

[7] Tohoku Univ, Grad Sch Life Sci, Sendai, Miyagi 9808577, Japan

[8] Kyoto Sangyo Univ, Kita Ku, Kyoto 6038555, Japan

[9] Univ Missouri, Div Plant Sci, Columbia, MO 65211 USA

[10] Univ Missouri, Div Biochem, Columbia, MO 65211 USA

[11] Mitsubishi Mat Corp, Met Co, Met Dev Ctr, Naoshima, Kagawa 7613110, Japan

来源：

PLANT JOURNAL | 2018年 / 93卷 / 01期

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

aspartic peptidase; late nodulin; Lotus japonicus; nodule; restriction of symbionts; rhizobia-legume symbiosis; THAUMATIN-LIKE PROTEIN; MEDICAGO-TRUNCATULA; PERIBACTEROID MEMBRANE; EARLY SENESCENCE; RHIZOBIUM-LOTI; ROOT-NODULES; LEGUME; FIXATION; GENE; PLANT;

D O I：

10.1111/tpj.13759

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The nitrogen-fixing symbiosis of legumes and Rhizobium bacteria is established by complex interactions between the two symbiotic partners. Legume Fix(-) mutants form apparently normal nodules with endosymbiotic rhizobia but fail to induce rhizobial nitrogen fixation. These mutants are useful for identifying the legume genes involved in the interactions essential for symbiotic nitrogen fixation. We describe here a Fix(-) mutant of Lotus japonicus, apn1, which showed a very specific symbiotic phenotype. It formed ineffective nodules when inoculated with the Mesorhizobium loti strain TONO. In these nodules, infected cells disintegrated and successively became necrotic, indicating premature senescence typical of Fix(-) mutants. However, it formed effective nodules when inoculated with the M. loti strain MAFF303099. Among nine different M. loti strains tested, four formed ineffective nodules and five formed effective nodules on apn1 roots. The identified causal gene, ASPARTIC PEPTIDASE NODULE-INDUCED 1 (LjAPN1), encodes a nepenthesin-type aspartic peptidase. The well characterized Arabidopsis aspartic peptidase CDR1 could complement the strain-specific Fix(-) phenotype of apn1. LjAPN1 is a typical late nodulin; its gene expression was exclusively induced during nodule development. LjAPN1 was most abundantly expressed in the infected cells in the nodules. Our findings indicate that LjAPN1 is required for the development and persistence of functional (nitrogen-fixing) symbiosis in a rhizobial strain-dependent manner, and thus determines compatibility between M. loti and L. japonicus at the level of nitrogen fixation.

引用

页码：5 / 16

页数：12