Efficient and direct glutathione production from raw starch using engineered Saccharomyces cerevisiae

被引：13

作者：

Yoshida, Hideyo ^{[1
]}

Arai, Shogo ^{[1
]}

Hara, Kiyotaka Y. ^{[2
]}

Yamada, Ryosuke ^{[1
]}

Ogino, Chiaki ^{[1
]}

Fukuda, Hideki ^{[1
]}

Kondo, Akihiko ^{[1
]}

机构：

[1] Kobe Univ, Grad Sch Engn, Dept Sci & Chem Engn, Nada Ku, Kobe, Hyogo 6578501, Japan

[2] Kobe Univ, Org Adv Sci & Technol, Nada Ku, Kobe, Hyogo 6578501, Japan

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2011年 / 89卷 / 05期

关键词：

Glutathione; Yeast; Amylase; Starch; Biorefinery; YEAST; FERMENTATION; ETHANOL; ANTIOXIDANT; LIMITATION; TOXICITY; CELLS;

D O I：

10.1007/s00253-010-2968-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Glutathione is a valuable tri-peptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is produced industrially by fermentation using Saccharomyces cerevisiae. We demonstrated that expression of amylase genes in glutathione-producing S. cerevisiae enables direct use of starch as a carbon source, thus eliminating the Crabtree effect that is caused by excess glucose. Consequently, cell growth and glutathione productivity were significantly improved. This approach is potentially applicable to a variety of fermentative processes for production of value-added chemicals under aerobic conditions.

引用

页码：1417 / 1422

页数：6

共 21 条

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