Characterisation and modification of the exopolysaccharide produced by Lactococcus lactis subsp. cremoris B40

被引:52
作者
van Casteren, WHM
Dijkema, C
Schols, HA
Beldman, G
Voragen, AGJ
机构
[1] Wageningen Univ Agr, Dept Food Technol & Nutr Sci, Food Sci Grp, NL-6703 HD Wageningen, Netherlands
[2] Wageningen Univ Agr, Dept Biomol Sci, Mol Phys Lab, NL-6703 HA Wageningen, Netherlands
关键词
exopolysaccharide; Lactococcus; chemical structure; modification; phosphorus;
D O I
10.1016/S0144-8617(98)00044-7
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The chemical structure of an exopolysaccharide produced by Lactococcus lactis subsp. cremoris B40 is studied, explaining earlier reported analytical discrepancies. The EPS was found to have a molecular mass of 6.8 x 10(5) g mol(-1) and a molar ratio of rhamnose:galactose: glucose:phosphorus of 1:1.3:2:1.1. P-31 NMR indicated that a single phosphate group is present as a phosphodiester. EPS B40 was chemically modified using 0.6 M H2SO4, 28 M HF or 2 M NaOH. From these modifications it could be concluded that galactose I-phosphate was linked at the 3-position of 1,2,3,4-linked galactose in the backbone of the EPS. Furthermore, it appeared that during the hydrolysis step of the sugar composition analysis the galactose 3-phosphate linkages were only partially split and that, as a result, the amount of galactose was underestimated in presence of phosphate. Finally, it was demonstrated that a crude cellulase preparation was able to degrade dephosphorylated and partially de-rhamnosylated EPS. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:123 / 130
页数:8
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