Ultrastructural Changes in Cryptosporidium parvum Oocysts by Gamma Irradiation

被引：4

作者：

Joung, Migyo ^{[1
]}

Yun, Sejeong ^{[1
]}

Joung, Mijoung ^{[1
]}

Park, Woo-Yoon ^{[2
]}

Yu, Jae-Ran ^{[1
]}

机构：

[1] Konkuk Univ, Sch Med, Dept Environm & Trop Med, Seoul 143701, South Korea

[2] Chungbuk Natl Univ, Coll Med, Dept Radiat Oncol, Cheongju 361763, South Korea

来源：

KOREAN JOURNAL OF PARASITOLOGY | 2011年 / 49卷 / 01期

关键词：

Cryptosporidium parvum; ultrastructure; gamma irradiation; X-IRRADIATION; MEMBRANE; CELLS; INFECTIVITY; RADIATION; LYSOSOMES; CHARGE; WATER;

D O I：

10.3347/kjp.2011.49.1.25

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cryptosporidium parvum is known as one of the most highly resistant parasites to gamma irradiation. To morphologically have an insight on the radioresistance of this parasite, ultrastructural changes in C. parvum sporozoites were observed after gamma irradiation using various doses (1, 5, 10, and 25 kGy) following a range of post-irradiation incubation times (10 kGy for 6, 12, 24, 48, 72, and 96 hr). The ultrastructures of C. parvum oocysts changed remarkably after a 10-kGy irradiation. Nuclear membrane changes and degranulation of dense granules were observed with high doses over 10 kGy, and morphological changes in micronemes and rhoptries were observed with very high doses over 25 kGy. Oocyst walls were not affected by irradiation, whereas the internal structures of sporozoites degenerated completely 96 hr post-irradiation using a dose of 10 kGy. From this study, morphological evidence of radioresistance of C. parvum has been supplemented.

引用

页码：25 / 31

页数：7

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