Regeneration of Echinacea purpurea:: Induction of root organogenesis from hypocotyl and cotyledon explants

被引:30
作者
Choffe, KL [1 ]
Murch, SJ [1 ]
Saxena, PK [1 ]
机构
[1] Univ Guelph, Dept Plant Agr, Div Biotechnol, Guelph, ON N1G 2W1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
Echinacea purpurea L; indoleacetic acid; indolebutyric acid; naphthaleneacetic acid; root regeneration;
D O I
10.1023/A:1006444821769
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An in vitro propagation system was developed for Echinacea purpurea L. (purple coneflower), a medicinal plant commonly used in the treatment of colds, flu and related ailments. Root organogenesis from Echinacea purpurea hypocotyl explants was effectively induced by indolebutyric acid. Indoleacetic acid was found to be less effective than indolebutyric acid while treatments with naphthaleneacetic acid were ineffective for induction of root organogenesis. The results of this study have established a micropropagation system for Echinacea purpurea that will provide axenic plant material for further investigations into medicinally active biochemicals and the mass production of high-quality Echinacea purpurea root tissues for the commercial market.
引用
收藏
页码:227 / 234
页数:8
相关论文
共 21 条
[1]  
BETZ W, 1998, SCI REV ALT MED, V2, P12
[2]  
Choffe KL, 2000, IN VITRO CELL DEV-PL, V36, P30
[3]  
CHOI KT, 1988, BIOTECHNOLOGY AGR FO, V4
[4]  
CON, 2000, CONSUMER REPORT MAY, P17
[5]  
CURTIN ME, 1983, BIO-TECHNOL, V1, P649
[6]   Effectiveness of indoleacetic acid, indolebutyric acid and naphthaleneacetic acid during adventitious root formation in vitro in Malus 'Jork 9' [J].
DeKlerk, GJ ;
TerBrugge, J ;
Marinova, S .
PLANT CELL TISSUE AND ORGAN CULTURE, 1997, 49 (01) :39-44
[7]   FORMATION OF PLANTLETS FROM CALLUS-CULTURES OF GINSENG (PANAX-GINSENG) [J].
FURUYA, T ;
YOSHIKAWA, T ;
USHIYAMA, K ;
ODA, H .
EXPERIENTIA, 1986, 42 (02) :193-194
[8]   NUTRIENT REQUIREMENTS OF SUSPENSION CULTURES OF SOYBEAN ROOT CELLS [J].
GAMBORG, OL ;
MILLER, RA ;
OJIMA, K .
EXPERIMENTAL CELL RESEARCH, 1968, 50 (01) :151-+
[9]  
HOBBS C, 1989, ECHINACEA HDB
[10]   THE EFFECT OF FUNGAL COLONIZATION ON THE MORPHINE PRODUCTION OF POPPY (PAPAVER-SOMNIFERUM L) CAPSULES [J].
LAUGHLIN, JC ;
MUNRO, D .
JOURNAL OF AGRICULTURAL SCIENCE, 1982, 98 (JUN) :679-&