In vitro studies of interaction of modified silica nanoparticles with different types of immunocompetent cells

被引：5

作者：

Kulikova, Galina A. ^{[1
]}

Parfenyuk, Elena V. ^{[1
]}

Ryabinina, Irina V. ^{[1
]}

Antsiferova, Yuliya S. ^{[2
]}

Sotnikova, Nataliya Yu. ^{[2
]}

Posiseeva, Lubov V. ^{[2
]}

Eliseeva, Mariya A. ^{[2
]}

机构：

[1] RAS, Sci Inst Solut Chem, Inst Russian Acad, Ivanovo 153045, Russia

[2] Fed Agcy High Med Technol, Fed State Inst Ivanovo Res Inst Matern & Childhoo, Ivanovo 153045, Russia

来源：

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2010年 / 95A卷 / 02期

关键词：

silica nanoparticles; sol-gel; flow cytometry; lymphocytes; macrophages; SECONDARY STRUCTURE; CONTROLLED-RELEASE; DELIVERY-SYSTEM; DRUG-DELIVERY; ADSORPTION; SIZE; FTIR;

D O I：

10.1002/jbm.a.32855

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Interactions between different types of immune cells and organically-modified silica nanoparticles were studied. The silica particles functionalized with amine groups were prepared by sol-gel technique. Sheep immunoglobulin labeled with fluoresceine isothiocyanate was immobilized by adsorption onto the nanoparticles. The presence of the functional groups was confirmed by infrared absorption measurements. The level of immunocompetent cells interacting with the silica nanoparticles was estimated as the amount of fluorescence-bright cells by flow cytometry method. A low level of interaction of the peripheral blood lymphocytes with the silica nanoparticles was found. On the contrary, the macrophages are actively involved in interaction with the silica nanoparticles. The influence of different size of the silica nanoparticles and incubation time on viability and functional activity of peripheral blood lymphocytes and peritoneal macrophages were investigated. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 95A: 434-439, 2010.

引用

页码：434 / 439

页数：6

共 21 条

[1] Silica xerogel carrier material for controlled release of toremifene citrate [J].

Ahola, M ;

Kortesuo, P ;

Kangasniemi, I ;

Kiesvaara, J ;

Yli-Urpo, A .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2000, 195 (1-2) :219-227

[2] ANTIMETASTATIC ACTIVITY OF MDP-L-ALANYL-CHOLESTEROL INCORPORATED INTO VARIOUS TYPES OF NANOCAPSULES [J].

BARRATT, G ;

PUISIEUX, F ;

YU, WP ;

FOUCHER, C ;

FESSI, H ;

DEVISSAGUET, JP .

INTERNATIONAL JOURNAL OF IMMUNOPHARMACOLOGY, 1994, 16 (5-6) :457-461

[3]

Boeckler C, 1999, EUR J IMMUNOL, V29, P2297, DOI 10.1002/(SICI)1521-4141(199907)29:07<2297::AID-IMMU2297>3.0.CO

[4]

2-5

[5] Stepwise growth of ultrathin SiOx films on Si(100) surfaces through sequential adsorption/oxidation cycles of alkylsiloxane monolayers [J].

Brunner, H ;

Vallant, T ;

Mayer, U ;

Hoffmann, H .

LANGMUIR, 1996, 12 (20) :4614-4617

[6] Changes in the secondary structure of adsorbed IgG and F(ab')(2) studied by FTIR spectroscopy [J].

Buijs, J ;

Norde, W ;

Lichtenbelt, JWT .

LANGMUIR, 1996, 12 (06) :1605-1613

[7] Adsorption of polyethyleneimine and polymethacrylic acid onto synthesized hematite [J].

Chibowski, S. ;

Patkowski, J. ;

Grzadka, E. .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2009, 329 (01) :1-10

[8] Nanotechnologies for drug delivery: Application to cancer and autoimmune diseases [J].

Couvreur, P. ;

Gref, R. ;

Andrieux, K. ;

Malvy, C. .

PROGRESS IN SOLID STATE CHEMISTRY, 2006, 34 (2-4) :231-235

[9] DETERMINATION OF THE SECONDARY STRUCTURE-CONTENT OF PROTEINS IN AQUEOUS-SOLUTIONS FROM THEIR AMIDE-I AND AMIDE-II INFRARED BANDS - COMPARISON BETWEEN CLASSICAL AND PARTIAL LEAST-SQUARES METHODS [J].

DOUSSEAU, F ;

PEZOLET, M .

BIOCHEMISTRY, 1990, 29 (37) :8771-8779

[10] Developments on drug delivery systems for the treatment of mycobacterial infections [J].

Gaspar, M. M. ;

Cruz, A. ;

Fraga, A. G. ;

Castro, A. G. ;

Cruz, M. E. M. ;

Pedrosa, J. .

CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2008, 8 (07) :579-591

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