Characterization of the second harmonic signal from collagen

被引：14

作者：

Cox, G ^{[1
]}

Xu, P ^{[1
]}

Sheppard, C ^{[1
]}

Rarnshaw, J ^{[1
]}

机构：

[1] Univ Sydney, Electron Microscope Unit, Sydney, NSW 2006, Australia

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES III | 2003年 / 4963卷

关键词：

second harmonic generation; collagen; non-linear microscopy; 2-photon fluorescence; colocalization;

D O I：

10.1117/12.485598

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Collagen is known to be a very effective generator of the second harmonic of incident light from 700 to 1100nm, and second harmonic generation (SHG) microscopy is coming into use as a tool for studying the distribution of collagen in tissue. It also shows promise as a technique for characterising collagen - both in distinguishing different collagen types and their packing and in identifying degradation of collagen in pathologic conditions. However many aspects of image formation in SHG microscopy of collagen remain imperfectly understood, and we have commenced a rigorous study of these factors. The present paper presents the first results from this program.

引用

页码：32 / 40

页数：9

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