Cryo-EM Structure of the African Swine Fever Virus

被引：167

作者：

Liu, Sheng ^{[1
,2
]}

Luo, Yuzi ^{[3
,4
]}

Wang, Yajuan ^{[5
]}

Li, Shihua ^{[2
]}

Zhao, Zhennan ^{[2
]}

Bi, Yuhai ^{[2
,6
]}

Sun, Junqing ^{[7
]}

Peng, Ruchao ^{[2
]}

Song, Hao ^{[8
]}

Zhu, Dongjie ^{[5
]}

Sun, Yuan ^{[3
,4
]}

Li, Su ^{[3
,4
]}

Zhang, Li ^{[3
,4
]}

Wang, Wei ^{[3
,4
]}

Sun, Yeping ^{[2
]}

Qi, Jianxun ^{[2
]}

Yan, Jinghua ^{[12
]}

Shi, Yi ^{[2
,6
,7
,9
]}

Zhang, Xinzheng ^{[5
]}

Wang, Peiyi ^{[7
,10
]}

Qiu, Hua-Ji ^{[3
,4
]}

Gao, George F. ^{[1
,2
,6
,7
,8
,9
,11
]}

机构：

[1] Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China

[2] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China

[3] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150069, Heilongjiang, Peoples R China

[4] Chinese Acad Agr Sci, Harbin Vet Res Inst, Natl High Containment Lab Anim Dis Control & Prev, Harbin 150069, Heilongjiang, Peoples R China

[5] Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China

[6] Chinese Acad Sci, Ctr Influenza Res & Early Warning CASCIRE, CAS TWAS Ctr Excellence Emerging Infect Dis CEEID, Beijing 100101, Peoples R China

[7] Shanxi Acad Adv Res & Innovat, Taiyuan 030032, Shanxi, Peoples R China

[8] Chinese Acad Sci, Beijing Inst Life Sci, RNIH, Beijing 100101, Peoples R China

[9] Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China

[10] Southern Univ Sci & Technol, SUSTech CryoEM Ctr, Shenzhen 518055, Guangdong, Peoples R China

[11] Chinese Ctr Dis Control & Prevent China CDC, Natl Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China

[12] Chinese Acad Sci, Inst Microbiol, CAS Key Lab Microbial Physiol & Metab Engn, Beijing, Peoples R China

来源：

CELL HOST & MICROBE | 2019年 / 26卷 / 06期

基金：

中国国家自然科学基金;

关键词：

MEMBRANE; FAUSTOVIRUS;

D O I：

10.1016/j.chom.2019.11.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

African swine fever virus (ASFV) is a large double-stranded DNA virus with an icosahedral multilayered structure. ASFV causes a lethal swine hemorrhagic disease and is currently responsible for widespread damage to the pork industry in Asia. Neither vaccines nor antivirals are available and the molecular characterization of the ASFV particle is outstanding. Here, we describe the cryogenic electron microscopy (cryo-EM) structure of the icosahedral capsid of ASFV at 4.6-angstrom. The ASFV particle consists of 8,280 copies of the major capsid protein p72, 60 copies of the penton protein, and at least 8,340 minor capsid proteins, of which there might be 3 different types. Like other nucleocytoplasmic large DNA viruses, the minor capsid proteins form a hexagonal network below the outer capsid shell, functioning as stabilizers by "gluing" neighboring capsomers together. Our findings provide a comprehensive molecular model of the ASFV capsid architecture that will contribute to the future development of countermeasures, including vaccines.

引用

页码：836 / +

页数：11

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