Limited spermatogenic differentiation of testicular tissue from prepubertal marmosets (Callithrix jacchus) in an in vitro organ culture system

被引：3

作者：

Sharma, Swati ^{[1
]}

Klaverkamp, Reinhild-Sandhowe ^{[1
]}

Wistuba, Joachim ^{[1
]}

Schlatt, Stefan ^{[1
]}

机构：

[1] Inst Reprod & Regenerat Biol, Ctr Reprod Med & Androl, Albert Schweitzer Campus 1,Bldg D11, D-48149 Munster, Germany

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2022年 / 539卷

关键词：

Primate; Marmoset; Testis; Puberty; Gonadotropins; Meiotic; TERM ORGANOTYPIC CULTURE; GERM-CELL DEVELOPMENT; TESTIS TISSUE; SURVIVAL; CRYOPRESERVATION; STRATEGY; INSIGHTS; SPERM; SERUM;

D O I：

10.1016/j.mce.2021.111488

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Purpose: of the research: To achieve male fertility preservation and restoration, experimental strategies for in vitro germ cell differentiation are required. The effects of two different culture conditions on in vitro maintenance and differentiation of non-human primate germ cells was studied. Three testes from three 6-month-old marmosets were cultured using a gas-liquid interphase system for 12 days. Testicular maturation in preculture control and samples cultured in gonadotropin and serum supplemented and non-supplemented culture samples was evaluated using Periodic Acid-Schiff (PAS) and immunohistochemical stainings. Principle results: Gonadotropins and serum-supplemented tissues demonstrate up to meiotic differentiation (BOULE + Pachytene spermatocyte) and advanced localization of germ cells (MAGEA4+). Moreover, complex (with gonadotropin and marmoset monkey serum) conditions induced progression in somatic cell maturation with advanced seminiferous epithelial organization, maintenance of encapsulation of cultured fragments with peritubular-myoid cells, preservation of tubular structural integrity and architecture. Major conclusions: We report stimulation-dependent in vitro meiotic transition in non-human primate testes. This model represents a novel ex vivo approach to obtain crucial developmental progression.

引用

页数：8

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