Comparison of spectrophotometric, HPLC and chemiluminescence methods for 3-nitrotyrosine and peroxynitrite interaction

被引：1
|
作者：
Turan, NN [1 ]
Ark, M
Demiryurek, AT
机构：
[1] Gazi Univ, Fac Pharm, Dept Pharmacol, TR-06330 Ankara, Turkey
[2] Mersin Univ, Fac Med, Dept Pharmacol, TR-33070 Mersin, Turkey
[3] Gaziantep Univ, Fac Med, Dept Pharmacol, TR-27310 Gaziantep, Turkey
来源：
ARCHIVES OF PHARMACAL RESEARCH | 2005年 / 28卷 / 03期
关键词：
3-nitrotyrosine; peroxynitrite; spectrophometric assay; chemiluminescence assay;
D O I：
10.1007/BF02977805
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration-dependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCl) was also studied via the chemiluminescence assay, where the HOCl-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine.
引用
收藏
页码：358 / 363
页数：6
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