Comparison of spectrophotometric, HPLC and chemiluminescence methods for 3-nitrotyrosine and peroxynitrite interaction

被引：1

作者：

Turan, NN ^{[1
]}

Ark, M

Demiryurek, AT

机构：

[1] Gazi Univ, Fac Pharm, Dept Pharmacol, TR-06330 Ankara, Turkey

[2] Mersin Univ, Fac Med, Dept Pharmacol, TR-33070 Mersin, Turkey

[3] Gaziantep Univ, Fac Med, Dept Pharmacol, TR-27310 Gaziantep, Turkey

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2005年 / 28卷 / 03期

关键词：

3-nitrotyrosine; peroxynitrite; spectrophometric assay; chemiluminescence assay;

D O I：

10.1007/BF02977805

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentration-dependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCl) was also studied via the chemiluminescence assay, where the HOCl-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine.

引用

页码：358 / 363

页数：6

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