Influence of nanoparticles surface coating on physicochemical properties for CRISPR gene delivery

被引：12

作者：

Alallam, Batoul ^{[1
]}

Doolaanea, Abd Almonem ^{[1
,3
]}

Oo, May Kyaw ^{[1
]}

Nasir, Mohd Hamzah Mohd ^{[2
]}

Taher, Muhammad ^{[1
]}

机构：

[1] Int Islamic Univ Malaysia, Dept Pharmaceut Technol, Kulliyyah Pharm, Kuantan 25200, Malaysia

[2] Int Islamic Univ Malaysia, Dept Biotechnol, Kulliyyah Sci, Kuantan 25200, Malaysia

[3] Int Islamic Univ Malaysia, IKOP Sdn Bhd, Kulliyyah Pharm, Kuantan 25200, Malaysia

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2021年 / 66卷

关键词：

Coating; Electrospray; Gene editing; Gene delivery; Alginate; Chitosan; Nanoparticle; CRISPR; CELLULAR UPTAKE; PARTICLE-SIZE; CHITOSAN; RELEASE; CELLS; CHALLENGES; STRATEGIES; THERAPY;

D O I：

10.1016/j.jddst.2021.102910

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

CRISPR is a powerful gene editing tool, but its non-viral delivery approaches are still limited due to low efficacy. To improve the efficacy of plasmid loaded alginate nanoparticles, three different polymer coating, chitosan, gum Arabic and PEG were applied and studied in comparison. The surface coating highly affected physicochemical properties of the nanoparticles. Smaller size nanoparticles showed higher cellular uptake in terms of quantity and efficiency. The transfection efficiency was highly correlated with the plasmid DNA released from the nanoparticles and the nanoparticles ability to protect the plasmid DNA from degradation. The indel gene editing efficiency of the nanoparticles was related to their protection ability but not related to their transfection efficiency. These results indicated the importance of careful tuning of nanomaterial physicochemical properties as it plays the ultimate role of the carrier biological activities.

引用

页数：17

共 56 条

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