Multiplex real-time PCR for the detection and quantification of DNA from beef, pork, horse and sheep

被引：95

作者：

Koeppel, Rene ^{[1
]}

Ruf, Juerg ^{[2
]}

Rentsch, Juerg ^{[3
]}

机构：

[1] Official Food Control Author Canton Zurich, Zurich, Switzerland

[2] Official Food Control Author Canton Thurgau, Thurgau, Switzerland

[3] Swiss Qual Testing Serv SQTS, Courtepin, Switzerland

来源：

EUROPEAN FOOD RESEARCH AND TECHNOLOGY | 2011年 / 232卷 / 01期

关键词：

Multiplex; Real-time quantitative PCR; Beef; Pork; Horse; Sheep; POLYMERASE-CHAIN-REACTION; SPECIES IDENTIFICATION; MEAT-PRODUCTS; QUANTITATION; CHICKEN; TURKEY; FOODS; ASSAY; DUCK;

D O I：

10.1007/s00217-010-1371-y

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Meat products are often composed of more than one meat species. A quantitative multiplex PCR was developed to determine the proportion of meat fractions of beef, pork, horse and sheep. The precision and accuracy were investigated by dilutions of DNA from all four species and examining different meat products from the market. Application of this tetraplex quantitative real-time PCR system will enable official food control and production control laboratories to efficiently investigate the composition of meat products.

引用

页码：151 / 155

页数：5

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