Extract of cochinchina momordica seeds regulates angiogenesis of rheumatoid arthritis through glycolysis inhibition

Objective: To observe the effects of extract of cochinchina momordica seeds (ECMS) on glucose metabolism, proliferation, migration, and tube formation for human umbilical vein endothelial cells (HUVEC), and to explore the effect and mechanism of ECMS on angiogenesis in rheumatoid arthritis. Methods: Alcohol extraction for ECMS by Soxhlet extractor; ECMS and 3-bromopyruvate (3-BrPa) act on cells. Cell counting kit-8 was used to detect cell proliferation, and Hoechst 33258 staining was used to detect cells apoptosis. Detection of cell hexokinase activity, glucose, lactate and ATP content was done through kits. Transwell detected cell migration, and vitro matrigel tube formation experiments were used to detect the tube number. Results: A volume of 200 mu g/mL ECMS and 40 mu g/mL 3-BrPa significantly inhibited the proliferation activity of HUVEC stimulated by TNF-alpha (10 nmol/L) (P<0.001), ECMS and 3-BrPa significantly inhibited TNF-alpha stimulation HUVEC hexokinase activity, reduced ATP and lactic acid content, as well as inhibited cell migration and tube formation ability. Conclusion: ECMS may regulate endothelial cell proliferation, migration and tube formation in vitro by inhibiting glycolysis, thereby inhibiting angiogenesis in rheumatoid arthritis.