Chemically Treated Strain Improvement of Aspergillus niger for Enhanced Production of Glucose Oxidase

被引：0

作者：

Zia, M. Anjum ^{[1
]}

Khalil-Ur-Rahman ^{[1
]}

Sheikh, Munir A. ^{[1
]}

Khan, Iftikhar A. ^{[2
]}

机构：

[1] Univ Agr Faisalabad, Dept Chem & Biochem, Faisalabad, Pakistan

[2] Univ Agr Faisalabad, Dept Plant Breeding & Genet, Faisalabad, Pakistan

来源：

INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY | 2010年 / 12卷 / 06期

关键词：

Glucose oxidase; Strain improvement; Mutagenesis; Aspergillus niger; PENICILLIUM-VARIABILE P16; MUTANTS; MUTAGENESIS; EXPRESSION; GENE;

D O I：

暂无

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The objective of research was to enhance the production of glucose oxidase through mutagenesis of Aspergillus niger Among colony restrictors, triton X-100 and ox-gall were used and it was found that 1% ox gall was the best In order to produce depressed mutants for enzyme production, 2-deoxy-D-glucose was used at 1 mg mL (1) PDA plates A few colonies were selected based on large clearance zones than wild type microorganism Glucose oxidase positive strain was identified on agar plate with o-dianisidine and peroxidase The size and color of zone is an index of the formation of glucose oxidase, giving rise a brown color The results indicated that A niger mutant BCM-8 and BCE-6 produced 9 and 6 mm enzyme diffusion zone with 282 and 202% increased activity, respectively (C) 2010 Friends Science Publishers

引用

页码：964 / 966

页数：3

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