A novel method for determination of peroxynitrite based on hemoglobin catalyzed reaction

被引：11
作者：
Liang, J [1 ]
Liu, ZH [1 ]
Cai, RX [1 ]
机构：
[1] Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China
来源：
ANALYTICA CHIMICA ACTA | 2005年 / 530卷 / 02期
基金：
中国国家自然科学基金;
关键词：
peroxynitrite; enzymatic method; hemoglobin; L-tyrosine; kinetics;
D O I：
10.1016/j.aca.2004.09.025
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel spectrofluorimetric method for the determination of peroxynitrite is proposed. The method is based on a mimetic enzyme catalyzed reaction with hemoglobin as the catalyst and L-tyrosine as the substrate. A new fluorescent substance is produced that might probably be the coupled dimmer of tyrosine, which, instead of nitryl-tyrosine, is likely to be a new marking substance of ONOO- injury in vivo. Kinetics of the reaction is studied and the possible reaction mechanism is also recommended. The proposed method is simple and highly sensitive with a detection limit of 5.00 x 10(-8) mol L-1 of peroxynitrite. A liner calibration graph is obtained over the peroxynitrite concentration range 5.60 x 10(-7) to 2.10 x 10(-5) mol L-1, with a correlation coefficient of 0.9983. Interferences from some amino acids and metal ions normally seen in biological samples, and also some anions structurally similar to ONOO- are studied. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：317 / 324
页数：8
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