Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800-850 of Rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls

被引：55

作者：

Bandilla, M

Ücker, B

Ram, M

Simonin, I

Gelhaye, E

McDermott, G

Cogdell, RJ

Scheer, H

机构：

[1] Univ Munich, Inst Bot, D-80638 Munchen, Germany

[2] Univ Glasgow, Div Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1998年 / 1364卷 / 03期

关键词：

photosynthesis; light harvesting complex; B800-850; B850; bacteriochlorophyll; pigment modification; pigment extraction; pigment exchange; reconstitution; reversible dissociation; circular dichroism;

D O I：

10.1016/S0005-2728(98)00086-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for reversibly removing bacteriochlorophyll from the B800-site of the B850-850 antenna complex from Rhodobacter sphaeroides. This method uses the oligosaccharidic detergent Triton BG-10, together with an incubation at pH 5.0, Reconstitution at the B800-site has been successfully achieved for a range of modified bacteriochlorophylls. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：390 / 402

页数：13

共 48 条

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