Reconstitution of the B800 bacteriochlorophylls in the peripheral light harvesting complex B800-850 of Rhodobacter sphaeroides 2.4.1 with BChl a and modified (bacterio-)chlorophylls

被引:55
作者
Bandilla, M
Ücker, B
Ram, M
Simonin, I
Gelhaye, E
McDermott, G
Cogdell, RJ
Scheer, H
机构
[1] Univ Munich, Inst Bot, D-80638 Munchen, Germany
[2] Univ Glasgow, Div Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1998年 / 1364卷 / 03期
关键词
photosynthesis; light harvesting complex; B800-850; B850; bacteriochlorophyll; pigment modification; pigment extraction; pigment exchange; reconstitution; reversible dissociation; circular dichroism;
D O I
10.1016/S0005-2728(98)00086-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A method is described for reversibly removing bacteriochlorophyll from the B800-site of the B850-850 antenna complex from Rhodobacter sphaeroides. This method uses the oligosaccharidic detergent Triton BG-10, together with an incubation at pH 5.0, Reconstitution at the B800-site has been successfully achieved for a range of modified bacteriochlorophylls. (C) 1998 Elsevier Science B.V. All rights reserved.
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页码:390 / 402
页数:13
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