Modeling Photo-Bleaching Kinetics to Create High Resolution Maps of Rod Rhodopsin in the Human Retina

被引：7

作者：

Ehler, Martin ^{[1
]}

Dobrosotskaya, Julia ^{[2
]}

Cunningham, Denise ^{[3
]}

Wong, Wai T. ^{[4
]}

Chew, Emily Y. ^{[5
]}

Czaja, Wojtek ^{[6
]}

Bonner, Robert F. ^{[7
]}

机构：

[1] Univ Vienna, Fac Math, Vienna, Austria

[2] Case Western Reserve Univ, Dept Math Appl Math & Stat, Cleveland, OH 44106 USA

[3] NEI, Off Clin Director, NIH, Bethesda, MD 20892 USA

[4] NEI, Unit Neuron Glia Interact, NIH, Bethesda, MD 20892 USA

[5] NEI, Div Epidemiol & Clin Applicat, NIH, Bethesda, MD 20892 USA

[6] Univ Maryland, Dept Math, College Pk, MD 20742 USA

[7] NICHHD, Sect Med Biophys, NIH, Bethesda, MD 20892 USA

来源：

PLOS ONE | 2015年 / 10卷 / 07期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

FUNDUS AUTOFLUORESCENCE; DARK-ADAPTATION; MACULAR PIGMENT; GEOGRAPHIC ATROPHY; BLOOD-VESSELS; IMAGES; SEGMENTATION; RECOVERY; DENSITY; LIGHT;

D O I：

10.1371/journal.pone.0131881

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We introduce and describe a novel non-invasive in-vivo method for mapping local rod rhodopsin distribution in the human retina over a 30-degree field. Our approach is based on analyzing the brightening of detected lipofuscin autofluorescence within small pixel clusters in registered imaging sequences taken with a commercial 488nm confocal scanning laser ophthalmoscope (cSLO) over a 1 minute period. We modeled the kinetics of rhodopsin bleaching by applying variational optimization techniques from applied mathematics. The physical model and the numerical analysis with its implementation are outlined in detail. This new technique enables the creation of spatial maps of the retinal rhodopsin and retinal pigment epithelium (RPE) bisretinoid distribution with an approximate to 50 mu m resolution.

引用

页数：22

共 69 条

[1] DENSITY AND PHOTOSENSITIVITY OF HUMAN RHODOPSIN IN LIVING RETINA [J].