Regulation of Vibrio mimicus metalloprotease (VMP) production by the quorum-sensing master regulatory protein, LuxR

被引：8
作者：
Abdel-Sattar, El-Shaymaa [1 ,2 ]
Miyoshi, Shin-ichi [1 ]
Elgaml, Abdelaziz [1 ,3 ]
机构：
[1] Okayama Univ, Grad Sch Med Dent & Pharmaceut Sci, Kita Ku, 1-1-1 Tsushima Naka, Okayama 7008530, Japan
[2] Chem Ind Dev Co, Assiut, Egypt
[3] Mansoura Univ, Dept Microbiol & Immunol, Fac Pharm, Elgomhouria St, Mansoura, Egypt
来源：
JOURNAL OF BASIC MICROBIOLOGY | 2016年 / 56卷 / 10期
关键词：
Vibrio mimicus; Metalloprotease; Quorum-sensing; LuxR protein; GENE-EXPRESSION; STRESS-RESPONSE; VULNIFICUS; HARVEYI; CHOLERAE; BINDING; SYSTEMS; SEQUENCE; LUMINESCENCE; TEMPERATURE;
D O I：
10.1002/jobm.201600002
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Vibrio mimicus is an estuarine bacterium, while it can cause severe diarrhea, wound infection, and otitis media in humans. This pathogen secretes a relatively important toxin named V. mimicus metalloprotease (VMP). In this study, we clarified regulation of the VMP production according to the quorum-sensing master regulatory protein named LuxR. First, the full length of luxR gene, encoding LuxR, was detected in V. mimicus strain E-37, an environmental isolate. Next, the putative consensus binding sequence of LuxR protein could be detected in the upstream (promoter) region of VMP encoding gene, vmp. Finally, the effect of disruption of luxR gene on the expression of vmp and production of VMP was evaluated. Namely, the expression of vmp was significantly diminished by luxR disruption and the production of VMP was severely altered. Taken together, here we report that VMP production is under the positive regulation of the quorum-sensing master regulatory protein, LuxR.
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页码：1051 / +
页数：8
相关论文
共 33 条
[1]   EXISTENCE OF A NOVEL HEMAGGLUTININ HAVING NO PROTEASE ACTIVITY IN VIBRIO-MIMICUS [J].
ALAM, M ;
MIYOSHI, S ;
MARUO, I ;
OGAWA, C ;
SHINODA, S .
MICROBIOLOGY AND IMMUNOLOGY, 1994, 38 (06) :467-470
[2]   A mutational analysis defines Vibrio fischeri LuxR binding sites [J].
Antunes, Luis Caetano M. ;
Ferreira, Rosana B. R. ;
Lostroh, C. Phoebe ;
Greenberg, E. Peter .
JOURNAL OF BACTERIOLOGY, 2008, 190 (13) :4392-4397
[3]  
Ausbel F. M., 1987, CURRENT PROTOCOLS MO
[4]   INTERCELLULAR SIGNALING IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES REGULATING EXPRESSION OF LUMINESCENCE [J].
BASSLER, BL ;
WRIGHT, M ;
SHOWALTER, RE ;
SILVERMAN, MR .
MOLECULAR MICROBIOLOGY, 1993, 9 (04) :773-786
[5]   MULTIPLE SIGNALING SYSTEMS CONTROLLING EXPRESSION OF LUMINESCENCE IN VIBRIO-HARVEYI - SEQUENCE AND FUNCTION OF GENES ENCODING A 2ND SENSORY PATHWAY [J].
BASSLER, BL ;
WRIGHT, M ;
SILVERMAN, MR .
MOLECULAR MICROBIOLOGY, 1994, 13 (02) :273-286
[6]   VASCULAR-PERMEABILITY ENHANCEMENT BY VIBRIO-MIMICUS PROTEASE AND THE MECHANISMS OF ACTION [J].
CHOWDHURY, MAR ;
MIYOSHI, S ;
SHINODA, S .
MICROBIOLOGY AND IMMUNOLOGY, 1991, 35 (12) :1049-1058
[7]   Role of the Histone-Like Nucleoid Structuring Protein (H-NS) in the Regulation of Virulence Factor Expression and Stress Response in Vibrio vulnificus [J].
Elgaml, Abdelaziz ;
Miyoshi, Shin-Ichi .
BIOCONTROL SCIENCE, 2015, 20 (04) :263-274
[8]   Presence of Nitric Oxide-Sensing Systems in the Human Pathogen Vibrio vulnificus [J].
Elgaml, Abdelaziz ;
Miyoshi, Shin-ichi .
BIOCONTROL SCIENCE, 2015, 20 (03) :199-203
[9]   Effects of temperature, growth phase and luxO-disruption on regulation systems of toxin production in Vibrio vulnificus strain L-180, a human clinical isolate [J].
Elgaml, Abdelaziz ;
Higaki, Kazutaka ;
Miyoshi, Shin-ichi .
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2014, 30 (02) :681-691
[10]   Regulation system of serine protease production in Vibrio vulnificus strain NCIMB 2137, a metalloprotease-gene negative strain isolated from a diseased eel [J].
Elgaml, Abdelaziz ;
Higaki, Kazutaka ;
Miyoshi, Shin-ichi .
AQUACULTURE, 2013, 416 :315-321
1234