Gold nanoparticle labeling of cells is a sensitive method to investigate cell distribution and migration in animal models of human disease

The ability to track cells in small-animal models of human disease is important because it gives the potential to improve our understanding of the processes of disease progression as well as our understanding of the therapeutic effects of interventions. In this study gold nanoparticles have been used as a permanent marker of implanted normal and malignant cell grafts in combination with a suitable x-ray apparatus. Using x-ray computed tomography the micrometric three-dimensional distribution of these marked cells could be displayed with penetration depth, high cell sensitivity and high spatial resolution in rodent models of human diseases. In principle the method allows quantification of cell numbers at any anatomical location over time in small animals. From the Clinical Editor: In this paper, a gold nanoparticle based cell labeling method is presented for in vivo cell tracking via micro-CT. Although a tumor model is shown in this pilot experiment, the method can theoretically be utilized in cell labeling experiments of any kind. (C) 2011 Elsevier Inc. All rights reserved.