Two-photon bioimaging with a multi-kilowatt peak-power optical pulse source using a 1-μm wavelength mode-locked semiconductor laser

被引：1

作者：

Guo, Hengchang ^{[1
]}

Takashima, Keijiro ^{[1
]}

Sato, Aya ^{[1
]}

Yokoyama, Hiroyuki ^{[1
]}

Mure, Masahito ^{[2
]}

Iseki, Yuji ^{[2
]}

Tsubokawa, Hiroshi ^{[3
]}

机构：

[1] Tohoku Univ, New Ind Creat Hatchery Ctr, Aoba Ku, 6-6-10 Aramaki Aoba, Sendai, Miyagi 9808579, Japan

[2] Sumitomo Osaka Cement Co Ltd, New Technol Res Labs, Funabashi, Chiba 2748601, Japan

[3] Tohoku Univ, Grad Sch Informat Sci, Aoba Ku, Sendai, Miyagi 9808579, Japan

来源：

MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES VIII | 2008年 / 6860卷

基金：

日本科学技术振兴机构; 日本学术振兴会;

关键词：

semiconductor lasers; fluorescence microscopy; multiphoton processes; medical and biological imaging; picosecond phenomena; fiber amplifiers; fluorescent proteins;

D O I：

10.1117/12.762922

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

We have developed a multi-kilowatt peak power 1-mu m optical pulse source for two-photon microscopy. Utilizing an external-cavity hybrid mode-locked semiconductor laser, we were able to generate picosecond optical pulses at a 500-MHz repetition rate. With a semiconductor optical amplifier driven by synchronized electronic gating pulses, the optical pulse repetition rate was sub-harmonically extracted at 1-100 MHz. At a 10-MHz repetition rate, optical pulses were then amplified to a peak power of greater than 2 kW with a two-stage Yb-doped fiber amplifier. Using this light source, we successfully obtained clear two-photon images of mouse brain neurons expressing green fluorescent proteins.

引用

页数：8

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