ISOTRETINOIN ANTI-ACNE GEL FOR THE MANAGEMENT OF P. ACNE INFECTION

The object of the paper is the development of anti-acne gel of isotretinoin and evaluated for as potential formulation to treat Acne vulgaris. The isotretinoin based anti-acne gel was prepared by using carbopol 940 polymers and evaluated for solubility, drug interaction, drug release, pH, viscosity and spreadability. In-vitro drug release through Franz diffusion cells, acute skin irritation test and antibacterial test also executed to confirm the potency of the gel formulation. The evaluation test was also compared with marketed formulation Sortet gel. The antibacterial (anti-acne) activity of different formulations was evaluated by the modified agar well diffusion method in the culture of Propionibacterium acne. The optimized formulation ISG-7 has shown the highest spreadability (42.422 g/cm(3)) with respect to other gel formulations and a high percentage of drug contents (96.42%). In-vitro diffusion study suggested that ISG-2, ISG-3, and ISG-7 have shown more diffusion and drug release from all the formulations that is 82.97%. 79.20% and 83.69% as compared to Sotret gel (86.72%). The antibacterial activity was studied on anaerobic microorganism P. acne, compared with marketed Sortet gel. The optimized formulation has shown maximum zone of inhibition to P. acne and its well below to marketed formulations and standard benzyl peroxide gel. The anti-acne gel of isotretinoin was successfully formulated and evaluated by different parameters. The results indicate that the active component, that is, isotretinoin is more effective when subjected to gel formulations and produces effective anti-acne activity in the management of Acne vulgaris.