Structure and gene cluster of the O-antigen of Enterobacter cloacae G3422

被引：2
作者：
Perepelov, Andrei, V [1 ]
Filatov, Andrei, V [1 ]
Zhu, Wenxuan [2 ]
Shashkov, Alexander S. [1 ]
Wang, Min [2 ]
Guo, Xi [2 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Nankai Univ, TEDA, TEDA Inst Biol Sci & Biotechnol, Tianjin 300457, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2021年 / 510卷
基金：
俄罗斯科学基金会; 俄罗斯基础研究基金会;
关键词：
Enterobacter cloacae; Bacterial polysaccharide structure; Solvolysis; O-antigen; Lipopolysaccharide; O-antigen gene cluster; STRUCTURE GENERALIZATION SCHEME; STRUCTURE ELUCIDATION; ORGANIZATION; SEQUENCE; SUGAR;
D O I：
10.1016/j.carres.2021.108440
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3422 and studied by chemical methods, including sugar analyses, Smith degradation, and solvolysis with anhydrous trifluoroacetic acid, along with H-1 and C-13 NMR spectroscopy. The following structure of the branched tetrasaccharide repeating unit was established: alpha-L-6dTalp2,3,4OAc 1 down arrow 3 -> 4)-alpha-D-Manp-(1 -> 3)-alpha-D-Manp-(1 -> 3)-beta-D-GlcpNAc-(1 -> The O-antigen gene cluster of Enterobacter cloacae G3422 was sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in a good agreement with the O-antigen structure.
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页数：5
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