Enzyme-linked immune sorbent assay for PR-toxin in taxonomical assessment of fungi belonging to the genus Penicillium link

被引:5
作者
Burkin, A. A.
Kononenko, G. P. [1 ]
Kochkina, G. A.
Ozerskaya, S. M.
机构
[1] Russian Acad Agr Sci, All Russia Res Inst Vet Sanitat Hyg & Ecol, Moscow 123022, Russia
[2] Russian Acad Sci, All Russia Collect Microorganisms, Skryabin Inst Biochem & Physiol Microorganisms, Moscow 113184, Russia
基金
俄罗斯基础研究基金会;
关键词
D O I
10.1134/S0003683807040175
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The use of an indirect competitive enzyme-linked immune sorbent assay (ELISA) involving polyclonal rabbit antibodies against BSA-conjugated PR-toxin (sensitivity, 1 ng/ml) established the ability to synthesize PR-toxin in 18 out of 35 morphologically identified strains of Penicillium roqueforti and P. chrysogenum. The results indicate that ELISA for PR-toxin may be used in assessing the taxonomical position of terverticillate penicillia in the presence of other micotoxins.
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页码:453 / 458
页数:6
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